Sulfo-Cyanine5 carboxylic acid

Cat. # Quantity Price Lead time
13390 1 mg $110.00 in stock
23390 5 mg $290.00 in stock
43390 25 mg $690.00 in stock
53390 50 mg $1270.00 in stock
63390 100 mg $1990.00 in stock

Non-activated sulfo-Cyanine5 carboxylic acid, water soluble dye. This dye is highly hydrophilic and water-soluble. Non-sulfonated analog is available.

The fluorophore is an equivalent of Cy5® carboxylic acid.

For labeling applications consider using pre-activated sulfo-Cy5 NHS ester

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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Amino derivative of fluorescein, pure 5-isomer. Contains a linker arm between the aliphatic amine group, and the fluorophore. Useful for the labeling via enzymatic transamination, for the reactions with electrophilic reagents, and for reductive amination.

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

General properties

Appearance: dark blue powder
Molecular weight: 680,87
CAS number: 1121756-16-8, 1144162-77-5
Molecular formula: C32H37N2KO8S2
Solubility: Good in water, DMF, DMSO. Low in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Ranjit, S.; Malacrida, L.; Gratton, E. Differences between FLIM phasor analyses for data collected with the Becker and Hickl SPC830 card and with the FLIMbox card. Microscopy Research and Technique, 2018, 81(9), 980–989. doi: 10.1002/jemt.23061
  2. Razgoniaeva, N.; Rogers, S.; Moroz, P.; Cassidy, J.; Zamkov, M. Improving the spectral resolution in fluorescence microscopy through shaped-excitation imaging. Methods and Applications in Fluorescence, 2018, 6(4), 045006. doi: 10.1088/2050-6120/aad81c
  3. Siltanen, C.A.; Cole, R.H.; Poust, S.; Chao, L.; Tyerman, J.; Kaufmann-Malaga, B.; Ubersax, J.; Gartner, Z.J.; Abate, A.R. An Oil-Free Picodrop Bioassay Platform for Synthetic Biology. Scientific Reports, 2018, 8, 7913. doi: 10.1038/s41598-018-25577-4
  4. Zhao, Y.; Zhang, W.; Li, C. Optimization of the conical mirror design based on Monte Carlo simulations for fluorescence molecular tomography. SPIE Proceedings: Multimodal Biomedical Imaging XIII, 2018, 10487, 104870M. doi: 10.1117/12.2290665
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