EdU (5-ethynyl-2'-deoxyuridine)

Cat. # Quantity Price Lead time
10540 100 mg $120.00 5 days
20540 250 mg $195.00 in stock
30540 500 mg $345.00 21 days
40540 1 g $590.00 21 days

EdU (ethynyldeoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. Then, DNA containing ethynyl groups can be developed by Click chemistry reaction with various dye azides. Fluorescent DNA can be detected by microscopy, or cells can be sorted by FACS.

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General properties

Appearance: light beige to light brown solid
Molecular weight: 252.22
CAS number: 61135-33-9
Molecular formula: C11H12N2O5
Solubility: good in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
MSDS: Download
Product specifications

Product citations

  1. Gerosa, L.; Chidley, C.; Froehlich, F.; Sanchez, G.; Lim, S.K.; Muhlich, J.; Chen, J.-Y.; Baker, G.J.; Schapiro, D.; Shi, T.; Yi, L.; Nicora, C.D.; Claas, A.; Lauffenburger, D.A.; Qian, W.-J.; Wiley, H.S.; Sorger, P.K. Sporadic ERK pulses drive non-genetic resistance in drug-adapted BRAFV600E melanoma cells. bioRxiv, preprint. doi: 10.1101/762294
  2. Morais, C.M.; Cunha, P.P.; Melo, T.; Cardoso, A.M.; Domingues, P.; Domingues, M.R.; Pedroso de Lima, M.C.; Jurado, A.S. Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma. Human Molecular Genetics, in press. doi: 10.1093/hmg/ddz152
  3. Sharma, P.; Mesci, P.; Carromeu, C.; McClatchy, D.R.; Schiapparelli, L.; Yates, J.R.; Muotri, A.R.; Cline, H.T. Exosomes regulate neurogenesis and circuit assembly. Proceedings of the National Academy of Sciences of the U.S.A., 2019, 116(32), 16086–16094. doi: 10.1073/pnas.1902513116
  4. Hernandez-Segura, A.; Brandenburg, S.; Demaria, M. Induction and Validation of Cellular Senescence in Primary Human Cells. Journal of Visualized Experiments, 2018, 136, e57782. doi: 10.3791/57782
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