sulfo-Cyanine5 tetrazine
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133E0 | 1 mg | $125 | in stock | |
| 233E0 | 5 mg | $325 | in stock | |
| 333E0 | 10 mg |
$470
|
in stock | |
| 433E0 | 25 mg |
$850
|
in stock | |
| 533E0 | 50 mg |
$1490
|
in stock | |
| 633E0 | 100 mg |
$1990
|
in stock |
TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefins.
sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation-based labeling.
This reagent possesses good aqueous solubility and stability in biological environments.
Absorption and emission spectra of Sulfo-Cyanine5
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EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.Cyanine5.5 carboxylic acid
Cyanine5.5 dye, free acid form, unactivated. The dye can be considered non-reactive for most applications.AF 488 azide
AF 488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.General properties
| Appearance: | dark blue solid |
| Molecular weight: | 864.09 |
| Molecular formula: | C42H46KN7O7S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Comeo, E.; Goulding, J.; Lin, C.-Y.; Groenen, M.; Woolard, J.; Kindon, N. D.; Harwood, C. R.; Platt, S.; Briddon, S. J.; Kilpatrick, L. E.; Scammells, P. J.; Hill, S. J.; Kellam, B. Ligand-Directed Labeling of the Adenosine A1 Receptor in Living Cells. J. Med. Chem., 2024, 67(14), 12099–12117. doi: 10.1021/acs.jmedchem.4c00835
- Kerr, M. D.; McBride, D. A.; Johnson, W. T.; Chumber, A. K.; Najibi, A. J.; Seo, B. R.; Stafford, A. G.; Scadden, D. T.; Mooney, D. J.; Shah, N. J. Immune-Responsive Biodegradable Scaffolds for Enhancing Neutrophil Regeneration. Bioengineering & Translational Medicine, 2023, 8(1), e10309. doi: 10.1002/btm2.10309
- Kerr, M.D.; Johnson, W.T.; McBride, D.A.; Chumber, A.K.; Shah, N.J. Biodegradable scaffolds for enhancing vaccine delivery. Bioengineering & translational medicine, 2023, 8(6), e10591. doi: 10.1002/btm2.10591
Short link - lumiprobe.com/sh/p/2t
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