AF 488 TCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118H0 | 1 mg | $199 | in stock | |
| 218H0 | 5 mg |
$649
|
in stock | |
| 418H0 | 25 mg |
$1789
|
in stock |
Trans-cyclooctene (TCO) is one of the most used cycloalkenes for bioconjugation tasks. TCO readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term aqueous stability, which is important in low-concentration applications such as protein-protein conjugations, etc.
This TCO is a AF 488-containing derivative. AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
Absorption and emission spectra of AF 488
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Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.
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BDP® 581/591 tetrazine
BDP 581/591 is a borondipyrromethene dye with a tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.Biotin-PEG4-tetrazine
Biotin tetrazine is a reagent for copper-free click chemistry labeling with biotin, a well-known affinity probe to streptavidin.General properties
| Appearance: | orange powder |
| Molecular weight: | 989.27 |
| Molecular formula: | C48H70N6O12S2 |
| Solubility: | good in water, DMSO; moderate in acetonitrile |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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