EU (5-ethynyl uridine)

Cat. # Quantity Price Lead time
2439-25mg 25 mg $129.00 in stock
2439-500mg 500 mg $950.00 in stock

5-ethynyl uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.

EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyl uridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.

EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via Click Chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).

Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.

5-ethynyl uridine has an advantage over its analog, 5-bromouridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromouridine. Thus, the Сlick Сhemistry approach allows whole-mount staining of large samples like organs or tissue fragments.

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EdU (5-ethynyl-2'-deoxyuridine)

EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.

General properties

Appearance: off white solid
Molecular weight: 268.22
CAS number: 69075-42-9
Molecular formula: C11H12N2O6
IUPAC name: 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
MSDS: Download
Product specifications
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