EU (5-ethynyl uridine)
|Cat. #||Quantity||Price||Lead time||Buy this product|
|2439-25mg||25 mg||$129.00||in stock|
|2439-500mg||500 mg||$950.00||in stock|
5-ethynyl uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.
EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyl uridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.
EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via Click Chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.
5-ethynyl uridine has an advantage over its analog, 5-bromouridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromouridine. Thus, the Сlick Сhemistry approach allows whole-mount staining of large samples like organs or tissue fragments.
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|Appearance:||off white solid|
|Solubility:||good in water, DMSO, DMF|
|Quality control:||NMR 1H, HPLC-MS (95%)|
|Storage conditions:||Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.|