5-Ethynyl-uridine (EU)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2439-25mg | 25 mg |
$137.00
|
in stock | |
| 2439-100mg | 100 mg |
$487.00
|
in stock | |
| 2439-500mg | 500 mg |
$950.00
|
in stock |
5-Ethynyl-uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.
EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyluridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.
EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via click chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.
5-Ethynyl-uridine has an advantage over its analog, 5-bromo-uridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromo-uridine. Thus, the click chemistry approach allows whole-mount staining of large samples like organs or tissue fragments.
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General properties
| Appearance: | off white solid |
| Molecular weight: | 268.22 |
| CAS number: | 69075-42-9 |
| Molecular formula: | C11H12N2O6 |
| IUPAC name: | 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | Download |
| Product specifications |
Product citations
- Guo, X.-L.; Wang, Y.-D.; Liu, Y.-J.; Chu, L.; Zhu, H.; Hu, Y.; Wu, R.-Y.; Xie, H.-Y.; Yu, J.; Li, S.-P.; Xiong, C.-Y.; Li, R.-Y.; Ke, F.; Chen, L.; Chen, G.-Q.; Chen, L.; Bai, F.; Enver, T.; Li, G.-H.; Li, H.-F.; Hong, D.-L. Fetal Hepatocytes Protect the HSPC Genome via Fetuin-A. Nature, 2025, 637(8045), 402–411. doi: 10.1038/s41586-024-08307-x
- Cacioppo, R.; Gillis, A.; Shlamovitz, I.; Zeller, A.; Castiblanco, D.; Crisp, A.; Haworth, B.; Arabiotorre, A.; Abyaneh, P.; Bao, Y.; Sale, J. E.; Berry, S.; Vidaković, A. T. CRL3ARMC5 Ubiquitin Ligase and Integrator Phosphatase Form Parallel Mechanisms to Control Early Stages of RNA Pol II Transcription. Molecular Cell, 2024, 84(24), 4808-4823.e13. doi: 10.1016/j.molcel.2024.11.024
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