AF 343 (Coumarin)-X-carboxylic acid

Cat. # Quantity Price Lead time
12690 1 mg $105 in stock
22690 5 mg $165 in stock
42690 25 mg $285 in stock
52690 50 mg $495 in stock
62690 100 mg $880 in stock
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AF 343 (Coumarin) is a blue-emitting fluorophore used as a laser dye. The fluorophore can serve as a FRET donor for FAM (fluorescein).

Absorption and emission spectra of AF 343 (Coumarin)

Absorption and emission spectra of AF 343 (Coumarin)

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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

General properties

Appearance: yellow crystals
Molecular weight: 398.45
CAS number: 946123-11-1
Molecular formula: C22H26N2O5
IUPAC name: 6-({4-Oxo-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-5-yl}carbonylamino)hexanoic acid
Solubility: Well soluble in DMF, DMSO, moderately soluble in DCM, DCE, EtOAc, insoluble in diethyl ether.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24
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