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L-Homopropargylglycine (HPG) is a noncanonical amino acid analog of methionine that contains a terminal alkyne moiety. HPG-labeling is a fast, sensitive, non-toxic, and non-radioactive alternative to the traditional technique for detecting nascent protein synthesis.
HPG is the cell-permeable compound randomly incorporated into synthesizing protein instead of methionine during translation. The resulting alkyne-labeled full-length proteins can be detected via copper-catalyzed click reaction with fluorescent or biotin-labeled azides and used for subsequent microscopic imaging or purification tasks.
Baleva, M.V.; Piunova, U.; Chicherin, I.; Vasilev, R.; Levitskii, S.; Kamenski, P. Mitochondrial Protein SLIRP Affects Biosynthesis of Cytochrome c Oxidase Subunits in HEK293T Cells. International Journal of Molecular Sciences, 2024, 25(1), 93. doi: 10.3390/ijms25010093
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