AF 384 (PEP) azide

Cat. # Quantity Price Lead time
A4530 1 mg $110 in stock
B4530 5 mg $210 in stock
C4530 10 mg $310 in stock
D4530 25 mg $410 in stock
E4530 50 mg $695 in stock
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Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted

PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.

This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.

Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

Spectra of AF 384 (PEP) (phenylethynylpyrene) in ethanol

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General properties

Appearance: off white / yellowish solid
Molecular weight: 544.64
CAS number: 1807521-02-3
Molecular formula: C34H32N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR 1H (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 293; 362; 384
ε, L⋅mol−1⋅cm−1: 46300
Emission maximum, nm: 389
Fluorescence quantum yield: 0.64

Product citations

  1. Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
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