Pyrene azide 1

Cat. # Quantity Price Lead time
A1530 1 mg $110 5 days
B1530 5 mg $210 in stock
C1530 10 mg $310 in stock
D1530 25 mg $410 in stock
E1530 50 mg $695 in stock
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This product will be discontinued soon in favor of Pyrene azide 2

Pyrene is a polyaromatic hydrocarbon with strong short-wavelength fluorescence. Unlike other fluorescent dyes, polyaromatic hydrocarbons are fluorescent probes with a strong sensitivity to the microenvironment. Thus, its fluorescence is different in polar, and nonpolar environments. Other effects can also be observed.

When two pyrenes are in close proximity, they form excimers. Excimer formation can be easily observed, and quantitatively estimated using fluorescent spectra.

Pyrene azide is a reagent for easy pyrene click chemistry labeling of any alkyne-bearing molecule. It allows turning any molecule into a pyrene-bearing probe.

This azide contains a hydrophilic triethyleneglycol linker to mitigate intrinsic pyrene hydrophobicity and facilitate attachment to biomolecules in aqueous solutions.

Another pyrene azide for click chemistry labeling is also available: Pyrene azide 2.

Absorption and emission spectra of pyrene fluorophore

Absorption and emission spectra of pyrene fluorophore

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General properties

Appearance: off-white to yellowish solid
Molecular weight: 402.45
CAS number: 2135330-58-2
Molecular formula: C23H22N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR 1H (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 343; 326; 313; 276; 265; 242; 234
Emission maximum, nm: 377; 397

Product citations

  1. Semikolenova, O.; Sakovina, L.; Akhmetova, E.; Kim, D.; Vokhtantsev, I.; Golyshev, V.; Vorobyeva, M.; Novopashin, S.; Novopashina, D. Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles. International Journal of Molecular Sciences, 2021, 22(20), 10919. doi: 10.3390/ijms222010919
  2. Krasheninina, O.A.; Fishman, V.S.; Lomzov, A.A.; Ustinov, A.V.; Venyaminova, A.G. Postsynthetic On-Column 2′ Functionalization of RNA by Convenient Versatile Method. International Journal of Molecular Sciences, 2020, 21(14), 5127. doi: 10.3390/ijms21145127
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