Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with (methyl)cyclopropenes and strained non-benzo-fused cycloalkynes.
Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.
BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.
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General properties
Appearance:
dark red solid
Mass spec M+ increment:
611.4
Molecular weight:
727.64
Molecular formula:
C40H46N7BF4O
Solubility:
good in DCM, DMSO, DMF
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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