TET phosphoramidite, 6-isomer

Cat. # Quantity Price Lead time
C8160 100 mg –   in stock
D8160 250 mg $197 in stock
F8160 1 g $575 in stock
G8160 5 g $2265 in stock
H8160 10 g please inquire in stock
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TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.

TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).

TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).

5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.

Recommendations for using the reagent:

Condensation: 3 min.

Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C).

Absorption and emission spectra of TET

Absorption and emission spectra of TET

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General properties

Appearance: white solid foam
Molecular weight: 981.72
CAS number: 877049-90-6
Molecular formula: C46H54N3Cl4O10P
IUPAC name: 2',4,7,7'-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09

Oligo synthesis details

Diluent: anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).
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