DusQ1 CPG 500

Cat. # Quantity Price Lead time
11960 100 mg –   in stock
31960 500 mg $530.00 in stock
41960 1 g $990.00 in stock
51960 5 g $4455.00 in stock

This modified support with a pore size of 500 Å is intended for the synthesis of oligonucleotides of up to 50 bases in length modified with non-fluorescent DusQ1 quencher at the 3’ end.

DusQ1 dark quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It can be used for combined quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, Alexa Fluor™ 350, FAM™, Alexa Fluor™ 488, JOE™, VIC™, R6G, HEX™, TET™, CAL Fluor™ Gold 540, and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.

Absorption spectrum of DusQ1

Absorption spectrum of DusQ1

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General properties

Appearance: purple beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 35935
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 70−80
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