JOE phosphoramidite, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
|
C1160
/ Easily soluble lyophilised form in 8 mL septum
|
100 mg |
–
|
in stock | |
|
D1160
/ Easily soluble lyophilised form in 8 mL septum
|
250 mg |
$209.90
|
in stock | |
|
F1160
/ Easily soluble lyophilised form in 30 mL septum
|
1 g |
$779.90
|
in stock | |
| G1160 | 5 g |
$2230.00
|
in stock | |
| H1160 | 10 g |
$3799.00
|
in stock |
JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.
Absorption and emission spectra of JOE dye
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General properties
| Appearance: | colorless solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | good solubility in THF and DCM |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | 10 % THF in acetonitrile. |
| Coupling conditions: | coupling time 6 min |
| Deprotection conditions: | Standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30 % ammonium hydroxide/40 % aqueous methylamine 1:1 v/v) can be used with ~5 % of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at RT), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C). |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
Short link - lumiprobe.com/sh/p/4g
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