JOE phosphoramidite, 5-isomer

Oligonucleotide synthesis JOE Phosphoramidites

Cat. #	Quantity	Price	Lead time
C1160 / Easily soluble lyophilised form in 8 mL septum	100 mg	–	in stock
D1160 / Easily soluble lyophilised form in 8 mL septum	250 mg	$165	in stock
F1160 / Easily soluble lyophilised form in 30 mL septum	1 g	$575	in stock
G1160	5 g	$2230	in stock
H1160	10 g	$3300	in stock

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JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.

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Absorption and emission spectra of JOE dye

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Are there any differences between probes containing 5-JOE and 6-JOE isomers in probe-based qPCR?

General properties

Appearance:	colorless solid
Molecular weight:	972.88
Molecular formula:	C₄₈H₆₀N₃Cl₂O₁₂P
Solubility:	good in DCM, acetonitrile
Quality control:	NMR ¹H, ³¹P, HPLC-MS (95%), functional testing
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	533
ε, L⋅mol⁻¹⋅cm⁻¹:	75000
Emission maximum, nm:	554
Fluorescence quantum yield:	0.61
CF₂₆₀:	0.36
CF₂₈₀:	0.28

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.