JOE phosphoramidite, 5-isomer

Cat. # Quantity Price Lead time
C1160 100 mg –   in stock
D1160 250 mg $165 in stock
F1160 1 g $575 in stock
G1160 5 g $2230 in stock
H1160 10 g $3300 in stock
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JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.

Absorption and emission spectra of JOE dye

Absorption and emission spectra of JOE dye

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General properties

Appearance: colorless solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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