JOE phosphoramidite, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| F1160 | 1 g |
$480.00
|
5 days | |
| G1160 | 5 g |
$2150.00
|
in stock | |
| H1160 | 10 g |
$3890.00
|
21 days |
JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.
Absorption and emission spectra of JOE dye
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General properties
| Appearance: | colorless solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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