JOE phosphoramidite, 5-isomer

Cat. # Quantity Price Lead time
F1160 1 g $480.00 in stock
G1160 5 g $2150.00 in stock
H1160 10 g $3890.00 21 days
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JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.

Absorption and emission spectra of JOE dye

Absorption and emission spectra of JOE dye

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FAM Phosphoramidite, 6-Isomer

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JOE azide, 5- isomer

JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for Click chemistry

General properties

Appearance: colorless solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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