JOE azide, 5- isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 31130 | 500 uL, 10 mM/DMSO | please inquire | 10 days | |
| A1130 | 1 mg | $110.00 | in stock | |
| B1130 | 5 mg | $210.00 | in stock | |
| C1130 | 10 mg | $310.00 | in stock | |
| D1130 | 25 mg |
$410.00
|
in stock | |
| E1130 | 50 mg |
$695.00
|
in stock | |
| F1130 | 100 mg |
$1190.00
|
in stock |
JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.
JOE dye is often used in qPCR; oligo labeling can be achieved using Click chemistry. This is an azide derivative of JOE for Click chemistry, pure 5-isomer.
Absorption and emission spectra of JOE dye
Recommended protocol
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BDP FL azide
BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.TAMRA azide, 5-isomer
TAMRA fluorescent dye azide for Click chemistry.BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.General properties
| Appearance: | red solid |
| Mass spec M+ increment: | 586.1 |
| Molecular weight: | 587.37 |
| CAS number: | 1422178-11-7 |
| Molecular formula: | C26H20N4Cl2O8 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Product citations
- Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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