JOE azide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1130 | 1 mg | $110 | in stock | |
| B1130 | 5 mg | $210 | in stock | |
| C1130 | 10 mg | $310 | in stock | |
| D1130 | 25 mg |
$410
|
in stock | |
| E1130 | 50 mg |
$695
|
in stock | |
| F1130 | 100 mg |
$1190
|
in stock |
JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.
JOE dye is often used in qPCR; oligo labeling can be achieved using сlick chemistry. This is an azide derivative of JOE for сlick chemistry, pure 5-isomer.
Absorption and emission spectra of JOE dye
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JOE alkyne, 6-isomer
JOE dye alkyne derivative for copper-catalyzed click chemistry reaction with azides. JOE dye is widely used for nucleic acid labeling.Alkyne dT phosphoramidite
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
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Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.General properties
| Appearance: | red solid |
| Mass spec M+ increment: | 586.1 |
| Molecular weight: | 587.37 |
| CAS number: | 1422178-11-7 |
| Molecular formula: | C26H20N4Cl2O8 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Product citations
- Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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