JOE azide, 5- isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A1130 | 1 mg | $110.00 | in stock | |
| B1130 | 5 mg | $210.00 | in stock | |
| C1130 | 10 mg | $310.00 | in stock | |
| D1130 | 25 mg |
$410.00
|
in stock | |
| E1130 | 50 mg |
$695.00
|
in stock | |
| F1130 | 100 mg |
$1190.00
|
in stock |
JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.
JOE dye is often used in qPCR; oligo labeling can be achieved using Click chemistry. This is an azide derivative of JOE for Click chemistry, pure 5-isomer.
Absorption and emission spectra of JOE dye
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JOE xanthene dye phosphoramidite for oligo synthesis. Absorption and emission spectra for JOE are located between FAM and TAMRA channels.
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FAM azide, 5-isomer
FAM (fluorescein) azide for Click chemistry, pure 5-isomer.TAMRA azide, 5-isomer
TAMRA fluorescent dye azide for Click chemistry.General properties
| Appearance: | red solid |
| Mass spec M+ increment: | 586.1 |
| Molecular weight: | 587.37 |
| Molecular formula: | C26H20N4Cl2O8 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Product citations
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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