JOE azide, 5- isomer

Cat. # Quantity Price Lead time
A1130 1 mg $110.00 in stock
B1130 5 mg $210.00 in stock
C1130 10 mg $310.00 in stock
D1130 25 mg $410.00 in stock
E1130 50 mg $695.00 in stock
F1130 100 mg $1190.00 in stock

JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.

JOE dye is often used in qPCR; oligo labeling can be achieved using Click chemistry. This is an azide derivative of JOE for Click chemistry, pure 5-isomer.

Absorption and emission spectra of JOE dye

Absorption and emission spectra of JOE dye

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Cyanine3.5 azide

Cyanine3.5 azide for biomolecule labeling through Click Chemistry.

General properties

Appearance: red solid
Mass spec M+ increment: 586.1
Molecular weight: 587.37
Molecular formula: C26H20N4Cl2O8
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
CF260: 0.36
CF280: 0.28

Product citations

  1. Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
  2. Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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