DusQ 1 dT phosphoramidite

Cat. # Quantity Price Lead time
1452-100mg 100 mg $340 in stock
1452-500mg 500 mg $1390 in stock
1452-1g 1 g $2290 in stock
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DusQ 1 dT phosphoramidite is a modified oligo synthesis reagent bearing true dark quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 1 efficiently quench fluorescence by FRET quenching and through static quenching via formation of a ground state complex with the reporter dye, and typically used to construct qPCR probes with a quencher moiety.

Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 1 can be paired with reporter dyes emitting in the green to yellow region to form completely non-fluorescent combination. DusQ 1 has a broad absorption spectrum with maximum in a range 480–580 nm and works in conjunction with the commonly used fluorophores, e.g. FAM, TET, JOE, HEX, and Cyanine3.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

Absorption spectrum of DusQ 1

Absorption spectrum of DusQ 1

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TBTA ligand

TBTA is one of the most widely used water-insoluble ligands for copper-catalyzed azide-alkyne cycloadditions (CuAAC).

General properties

Appearance: black powder
Molecular weight: 1411.58
Molecular formula: C76H91N12O13P
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 27300

Oligo synthesis details

Coupling conditions: 6 min coupling time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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