DusQ2 phosphoramidite

Cat. # Quantity Price Lead time
1473-1g 1 g $590.00 21 days

DusQ2 is a black quencher dye for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher. This quencher is ideal for HEX, JOE, ROX, Cyanine5 and other dyes with the emission in orange and red part of the spectrum.

The primary phosphoramidite group couples with hydroxyls efficiently in oligonucleotide synthesis.

Absorption spectrum of DusQ2

Absorption spectrum of DusQ2

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General properties

Appearance: dark colored solid
Molecular weight: 997.08
Molecular formula: C54H61N8O9P
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
ε, L⋅mol−1⋅cm−1: 34000
CF260: 0.31
CF280: 0.26

Oligo synthesis details

Diluent: 20% THF / acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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