DusQ 2 dT phosphoramidite

Cat. # Quantity Price Lead time
1451-100mg 100 mg $340 in stock
1451-500mg 500 mg $1390 in stock
1451-1g 1 g $2290 in stock
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DusQ 2 dT phosphoramidite is a modified oligo synthesis reagent bearing completely non-fluorescent quencher nucleotide base typically used to internally label DNA oligonucleotide probe. DusQ 2 has large extinction coefficient, efficiently quench fluorescence for dyes emitting in the far red region, such as Cyanine5.

DusQ 2 is typically used to construct qPCR probes with a quencher moiety. Reagent contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges. DusQ 2 has a absorption spectrum in of red and near-infrared range and can be paired with reporter dyes that emit in the range 560–670 nm, e.g. TAMRA, ROX, Cyanine3, and Cyanine5.

Usage

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

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General properties

Appearance: black powder
Molecular weight: 1413.55
Molecular formula: C75H89N12O14P
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552

Oligo synthesis details

Coupling conditions: 6 min coupling time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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