Alkyne dT phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1897-100mg | 100 mg | $330.00 | in stock | |
| 1897-1g | 1 g |
$2590.00
|
in stock | |
| 1897-5g | 5 g |
$9900.00
|
in stock |
This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. This allows to prepare modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain, by virtue of copper catalyzed Click chemistry reaction with various azides.
This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.
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Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.dsGreen Gel Staining Solution, 10000×
dsGreen for staining dsDNA in gels.General properties
| Appearance: | off white solid |
| Mass spec M+ increment: | 437.1 |
| Molecular weight: | 877.96 |
| Molecular formula: | C48H56N5O9P |
| Solubility: | good in acetonitrile, DCM |
| Quality control: | NMR 1H, NMR 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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