Alkyne dT phosphoramidite

Alkynes Oligonucleotide synthesis Phosphoramidites Click chemistry

Cat. #	Quantity	Price	Lead time
1897-100mg	100 mg	$330 $195	in stock
1897-1g	1 g	$2590 $1250	in stock
1897-5g	5 g	$9900 $2850	in stock

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This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to the deoxyuridine fragment, and it does not interfere with the hybridization of the complementary strand. This allows the preparation of modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain by virtue of copper-catalyzed Click chemistry reaction with various azides.

This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.

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General properties

Appearance:	off white solid
Mass spec M+ increment:	437.1
Molecular weight:	877.96
Molecular formula:	C₄₈H₅₆N₅O₉P
Solubility:	good in acetonitrile, DCM
Quality control:	NMR ¹H, NMR ³¹P, HPLC-MS
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.