Sulfo-Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A3330 | 1 mg | $110.00 | in stock | |
| B3330 | 5 mg | $290.00 | in stock | |
| C3330 | 10 mg |
$470.00
|
in stock | |
| D3330 | 25 mg |
$690.00
|
in stock | |
| E3330 | 50 mg |
$1270.00
|
in stock | |
| F3330 | 100 mg |
$1990.00
|
in stock |
Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.
This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.
Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.
Sulfo-Cyanine5 absorbance and emission spectra
Recommended protocol
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FAM azide, 6-isomer
FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.Cyanine3 hydrazide
Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.General properties
| Appearance: | dark blue crystals |
| Molecular weight: | 746.87 |
| Molecular formula: | C35H43N6NaO7S2 |
| Solubility: | very high in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Yang, Z.; Joyner, A.L. YAP is involved in replenishment of granule cell progenitors following injury to the neonatal cerebellum. bioRxiv, preprint. doi: 10.1101/558742
- De France, K.J.; Babi, M.; Vapaavuori, J.; Hoare, T.; Moran-Mirabal, J.M.; Cranston, E.D. 2.5D Hierarchical Structuring of Nanocomposite Hydrogel Films Containing Cellulose Nanocrystals. ACS Applied Materials & Interfaces, 2019, 11(6), 6325–6335. doi: 10.1021/acsami.8b16232
- Maerle, A.V.; Simonova, M.A.; Pivovarov, V.D.; Voronina, D.V.; Drobyazina, P.E.; Trofimov, D.Y.; Alekseev, L.P.; Zavriev, S.K.; Ryazantsev, D.Y. Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR. PloS One, 2019, 14(1), e0209860. doi: 10.1371/journal.pone.0209860
- Marín-Caba, L.; Chariou, P.L.; Pesquera, C.; Correa-Duarte, M.A.; Steinmetz, N.F. Tobacco Mosaic Virus-Functionalized Mesoporous Silica Nanoparticles, a Wool-Ball-like Nanostructure for Drug Delivery. Langmuir, 2019, 35(1), 203–211. doi: 10.1021/acs.langmuir.8b03337
Cy® is a trademark of GE Healthcare.
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