Sulfo-Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A3330 | 1 mg | $110.00 | in stock | |
| B3330 | 5 mg | $290.00 | in stock | |
| C3330 | 10 mg |
$470.00
|
in stock | |
| D3330 | 25 mg |
$690.00
|
in stock | |
| E3330 | 50 mg |
$1270.00
|
in stock | |
| F3330 | 100 mg |
$1990.00
|
in stock |
Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.
This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.
Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.
Sulfo-Cyanine5 absorbance and emission spectra
Recommended protocol
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Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.Sulfo-Cyanine5 NHS ester
Water-soluble Cyanine 5 succinimide ester.TAMRA NHS ester, 5-isomer
Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.
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General properties
| Appearance: | dark blue crystals |
| Molecular weight: | 762.98 |
| Molecular formula: | C35H43N6KO7S2 |
| Solubility: | very high in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Cutie, S.; Lunn, D.; Huang, G.N. In vitro and in vivo roles of glucocorticoid and vitamin D receptors in the control of cardiomyocyte proliferative potential. bioRxiv, preprint. doi: 10.1101/755827
- Hinman, S.; Wang, Y.; Allbritton, N. Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models. ChemRxiv, preprint. doi: 10.26434/chemrxiv.9759605.v1
- Long, M.J.C; Zhao, Y.; Aye, Y. Clofarabine Commandeers the RNR-α-ZRANB3 Nuclear Signaling Axis. Cell Chemical Biology, 2020, 27(1), 122–133.e5. doi: 10.1016/j.chembiol.2019.11.012
- Zhao, Meng and Börner, Richard and Sigel, Roland K. O. and Freisinger, Eva. Site-Specific Dual-Color Labeling of Long RNAs. Methods in Molecular Biology, 2020, 2106, 253–270. doi: 10.1007/978-1-0716-0231-7_16
Cy® is a trademark of GE Healthcare.
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