Sulfo-Cyanine5 azide

sulfo-Cyanine5 Azides Microscopy
Cat. # Quantity Price Lead time
A3330 1 mg $110.00 in stock
B3330 5 mg $290.00 in stock
C3330 10 mg $470.00 in stock
D3330 25 mg $690.00 in stock
E3330 50 mg $1270.00 in stock
F3330 100 mg $1990.00 in stock
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Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.

This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.

Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.

Sulfo-Cyanine5 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine5 azide structure
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Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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Unactivated monofunctional Cy3.5 carboxylic acid.

FAM azide, 5-isomer

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General properties

Appearance: dark blue crystals
Molecular weight: 746.87
Molecular formula: C35H43N6NaO7S2
Solubility: very high in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Maerle, A.V.; Simonova, M.A.; Pivovarov, V.D.; Voronina, D.V.; Drobyazina, P.E.; Trofimov, D.Y.; Alekseev, L.P.; Zavriev, S.K.; Ryazantsev, D.Y. Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR. PloS One, 2019, 14(1), e0209860. doi: 10.1371/journal.pone.0209860
  2. Marín-Caba, L.; Chariou, P.L.; Pesquera, C.; Correa-Duarte, M.A.; Steinmetz, N.F. Tobacco Mosaic Virus-Functionalized Mesoporous Silica Nanoparticles, a Wool-Ball-like Nanostructure for Drug Delivery. Langmuir, 2019, 35(1), 203–211. doi: 10.1021/acs.langmuir.8b03337
  3. Pitek, A.S.; Hu, H.; Shukla, S.; Steinmetz, N.F. Cancer Theranostic Applications of Albumin-Coated Tobacco Mosaic Virus Nanoparticles. ACS Applied Materials & Interfaces, 2018, 10(46), 39468–39477. doi: 10.1021/acsami.8b12499
  4. Karas, J.; Turner, B.J.; Shabanpoor, F. The Assembly of Fluorescently Labeled Peptide-Oligonucleotide Conjugates via Orthogonal Ligation Strategies. Methods in Molecular Biology, 2018, 1282, 355–363. doi: 10.1007/978-1-4939-8651-4_22
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Cy® is a trademark of GE Healthcare.
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