Sulfo-Cyanine5 azide

Cat. # Quantity Price Lead time
A3330 1 mg $110.00 in stock
B3330 5 mg $290.00 in stock
C3330 10 mg $470.00 in stock
D3330 25 mg $690.00 in stock
E3330 50 mg $1270.00 in stock
F3330 100 mg $1990.00 in stock

Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.

This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.

Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue crystals
Molecular weight: 762.98
Molecular formula: C35H43N6KO7S2
Solubility: very high in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Yang, Z.; Joyner, A.L. YAP is involved in replenishment of granule cell progenitors following injury to the neonatal cerebellum. bioRxiv, preprint. doi: 10.1101/558742
  2. De France, K.J.; Badv, M.; Dorogin, J.; Siebers, E.; Panchal, V.; Babi, M.; Moran-Mirabal, J.; Lawlor, M.; Cranston, E.D.; Hoare, T. Tissue Response and Biodistribution of Injectable Cellulose Nanocrystal Composite Hydrogels. ACS Biomaterials Science & Engineering, 2019, 5(5), 2235–2246. doi: 10.1021/acsbiomaterials.9b00522
  3. Zemella, A.; Richter, T.; Thoring, L.; Kubick, S. A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties. Methods in Molecular Biology, 2019, 1947, 57–77. doi: 10.1007/978-1-4939-9121-1_4
  4. Liang, S.; Guan, Y.; Zhang, Y. Layer-by-Layer Assembly of Microgel Colloidal Crystals via Photoinitiated Alkyne–Azide Click Reaction. ACS Omega, 2019, 4(3), 5650–5660. doi: 10.1021/acsomega.9b00354
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