Sulfo-Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A3330 | 1 mg | $110.00 | in stock | |
| B3330 | 5 mg | $290.00 | in stock | |
| C3330 | 10 mg |
$470.00
|
in stock | |
| D3330 | 25 mg |
$690.00
|
in stock | |
| E3330 | 50 mg |
$1270.00
|
in stock | |
| F3330 | 100 mg |
$1990.00
|
in stock |
Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.
This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.
Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.
Sulfo-Cyanine5 absorbance and emission spectra
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Protein labeling buffer (with THPTA), 1.5x
Catalytic buffer for Click chemistry modification of proteins.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.General properties
| Appearance: | dark blue crystals |
| Molecular weight: | 762.98 |
| Molecular formula: | C35H43N6KO7S2 |
| Solubility: | very high in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Singh, A.; Jothiprakasam, T.; Raghavan, J.V.; Jhunjhunwala, S. Sterile Inflammation Alters Neutrophil Kinetics in Mice. bioRxiv, preprint. doi: 10.1101/2021.02.12.430891
- Toyonaga, T.; Keith, B.P.; Barrow, J.B.; Schaner, M.S.; Wolber, E.A.; Beasley, C.; Huling, J.; Wang, Y.; Allbritton, N.L.; Chaumont, N.; Sadiq, T.S.; Koruda, M.j.; Rahbar, R.; Furey, T.S.; Sethupathy, P.; Sheikh, S.Z. Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier integrity and is associated with a poor clinical outcome in Crohn's disease. bioRxiv, preprint. doi: 10.1101/2020.02.21.960070
- Hinman, S.; Wang, Y.; Allbritton, N. Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models. ChemRxiv, preprint. doi: 10.26434/chemrxiv.9759605.v1
- Rueda-Alaña, E.; García-Moreno, F. Time in Neurogenesis: Conservation of the Developmental Formation of the Cerebellar Circuitry. Brain, Behavior and Evolution, 2022, 97, 33-47. doi: 10.1159/000519068
Cy® is a trademark of GE Healthcare.
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