Sulfo-Cyanine5 azide

Cat. # Quantity Price Lead time
A3330 1 mg $110.00 in stock
B3330 5 mg $290.00 in stock
C3330 10 mg $470.00 in stock
D3330 25 mg $690.00 in stock
E3330 50 mg $1270.00 in stock
F3330 100 mg $1990.00 in stock
Delivery options for stock items

Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.

This reagent, as opposed to competing products, is supplied as an alkaline metal salt and not triethylammonium salt. Therefore, it is a powder which is easy to handle and dispense.

Sulfo-Cyanine5 is an analog of Cy5®, a very popular fluorophore, therefore this reagent is compatible to a wide range of standard fluorescent instrumentation such as imagers, plate readers, and microscopes.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.

General properties

Appearance: dark blue crystals
Molecular weight: 746.87
Molecular formula: C35H43N6NaO7S2
Solubility: very high in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Chen, L., Payumo, A.Y.; Hirose, K.; Bigley, R.B.; Lovas, J.; Kuruvilla, R.; Huang, G.N. Sympathetic Nerve Activity Promotes Cardiomyocyte Cell-Cycle Arrest And Binucleation. bioRxiv, 124347. doi: 10.1101/124347
  2. Franke, C.E.; Czapar, A.E.; Patel, R.B.; Steinmetz, N.F. Tobacco Mosaic Virus-Delivered Cisplatin Restores Efficacy in Platinum-Resistant Ovarian Cancer Cells. Molecular Pharmaceutics, in press. doi: 10.1021/acs.molpharmaceut.7b00466
  3. Gupta, S.; M-Redmond, T; Meng, F.; Tidball, A.; Akil, H.; Watson, S.; Parent, J.M.; Uhler, M.; others. Fibroblast Growth Factor 2 Regulates Activity and Gene Expression of Human Postmitotic Excitatory Neuron. Journal of Neurochemistry, 2018, 145(3), 188–203. doi: 10.1111/jnc.14255
  4. Gulati, N.M.; Pitek, A.S.; Czapar, A.E.; Stewart, P.L.; Steinmetz, N.F. The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition. Journal of Materials Chemistry B, 2018, 6(15), 2204–2216. doi: 10.1039/C7TB03106H
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