FAM-11-dCTP, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2713-50nmol | 50 nmol | $190.00 | in stock | |
| 2713-250nmol | 250 nmol |
$750.00
|
in stock | |
| 2713-500nmol | 500 nmol |
$1200.00
|
10 days | |
| 2713-1umol | 1 umol |
please inquire
|
in stock |
6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).
FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright emission in the green spectrum.
FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA-chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.
The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescence-labeled dCTP into the growing DNA chain.
Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.
Absorption and emission spectra of FAM
General properties
| Appearance: | yellow/orange solid |
| Molecular weight: | 1012.5 |
| Molecular formula: | C39H40N5Li3O20P3 |
| IUPAC name: | ((2R,3S,5R)-5-(4-amino-5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | good in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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