FAM-11-dCTP, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2713-50nmol | 50 nmol | $115 | in stock | |
| 2713-250nmol | 250 nmol | $325 | in stock | |
| 2713-500nmol | 500 nmol |
$500
|
in stock | |
| 2713-1umol | 1 umol |
$910
|
in stock |
6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).
FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.
FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.
The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.
Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.
Absorption and emission spectra of FAM
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| Appearance: | yellow to orange solid |
| Molecular weight: | 1012.5 |
| Molecular formula: | C39H40N5Li3O20P3 |
| IUPAC name: | ((2R,3S,5R)-5-(4-amino-5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | good in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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