DusQ 1 phosphoramidite

Cat. # Quantity Price Lead time
1470-100mg 100 mg $290.00 in stock
1470-500mg 500 mg $990.00 in stock
1470-1g 1 g $1690.00 in stock
1470-10g 10 g please inquire in stock

DusQ 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.

DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.


Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

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DusQ 2 is a black quencher dye phosphoramidite for the synthesis of dual-labeled probes. Useful for HEX, JOE, ROX, Cyanine5, and other dyes.
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Alkyne phosphoramidite, 5'-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for click chemistry.

HEX azide, 6-isomer

Azide of green-yellow emitting HEX dye for conjugation with oligonucleotides via [3+2]-dipolar cycloaddition.

General properties

Appearance: black powder
Molecular weight: 995.11
CAS number: 374591-94-3
Molecular formula: C55H63N8O8P
IUPAC name: Phosphoramidous acid, bis(1-​methylethyl)​-​, 2-​[[2-​[bis(4-​methoxyphenyl)​phenylmethoxy]​ethyl]​[4-​[[2-​methoxy-​5-​methyl-​4-​[(4-​methyl-​2-​nitrophenyl)​azo]​phenyl]​azo]​phenyl]​amino]​ethyl 2-​cyanoethyl ester (9CI)
Solubility: good in acetonitrile
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: 50% DCM in acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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