Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM, and sometimes used as a quencher for it.
Like other xanthenes, TAMRA exists as two isomers (5- and 6-), which have very similar spectral properties. This is an azide derivative of 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed Click chemistry, or with cycloalkynes with copper-free strain promoted alkyne azide cycloaddition (spAAc) reaction.
Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.
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Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.
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Water soluble thiol-reactive derivative of Sulfo-Cyanine3, a Cy3 analog.
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General properties
Appearance:
violet solid / solution
Mass spec M+ increment:
512.2
Molecular weight:
512.56
CAS number:
1192590-89-8
Molecular formula:
C28H28N6O4
Solubility:
Good in DMF, DMSO, alcohols, low in water
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.