TAMRA azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A8130 | 1 mg | $110.00 | in stock | |
| B8130 | 5 mg | $210.00 | in stock | |
| C8130 | 10 mg | $310.00 | in stock | |
| D8130 | 25 mg |
$410.00
|
in stock | |
| E8130 | 50 mg |
$695.00
|
in stock | |
| F8130 | 100 mg |
$1190.00
|
in stock |
Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM, and sometimes used as a quencher for it.
Like other xanthenes, TAMRA exists as two isomers (5- and 6-), which have very similar spectral properties. This is an azide derivative of 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed Click chemistry, or with cycloalkynes with copper-free strain promoted alkyne azide cycloaddition (spAAc) reaction.
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General properties
| Appearance: | violet solid / solution |
| Mass spec M+ increment: | 512.2 |
| Molecular weight: | 512.56 |
| CAS number: | 1192590-89-8 |
| Molecular formula: | C28H28N6O4 |
| Solubility: | Good in DMF, DMSO, alcohols, low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Product citations
- Chen, X.; Xu, J.; Wong, N.-K.; Zhong, S.; Yang, M.; Liu, Z.; Lu, Y.; Li, W.; Zhou, Y. Chemoproteomic profiling of cobalamin-independent methionine synthases in plant with a covalent probe. Journal of Agricultural and Food Chemistry, in press. doi: 10.1021/acs.jafc.0c03301
- Xu, Y.; Deng, Z.; Shi, Y.; Chen, X.; Xu, J.; Zhong, S.; Xiao, Y.; Wong, N.-K.; Zhou, Y. Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe. Analytical Chemistry, 2020, 92(6), 4419–4426. doi: 10.1021/acs.analchem.9b05327
- Zhang, S.; Spiegelman, N.A.; Lin, H. Global Profiling of Sirtuin Deacylase Substrates Using a Chemical Proteomic Strategy and Validation by Fluorescent Labeling. Methods in Molecular Biology, 2019, 2009, 137–147. doi: 10.1007/978-1-4939-9532-5_11
- Tang, G.; Liu, L.; Wang, X.; Pan, Z. Discovery of 7H-pyrrolo[2,3-d]pyrimidine derivatives as selective covalent irreversible inhibitors of interleukin-2-inducible T-cell kinase (Itk). European Journal of Medicinal Chemistry, 2019, 173, 167–183. doi: 10.1016/j.ejmech.2019.03.055
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