TAMRA azide, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A8130 | 1 mg | $110 | in stock | |
B8130 | 5 mg | $135 | in stock | |
C8130 | 10 mg | $210 | in stock | |
D8130 | 25 mg | $390 | in stock | |
E8130 | 50 mg | $595 | in stock | |
F8130 | 100 mg | $790 | in stock |
Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM and is sometimes used as a quencher of FAM.
Like other xanthenes, TAMRA exists as two isomers (5- and 6-) with very similar spectral properties. This is an azide derivative of the 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed click chemistry (CuAAC) or with cycloalkynes with copper-free strain-promoted alkyne azide cycloaddition (SPAAC) reaction.
Absorption and emission spectra of 6-TAMRA
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Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 6-isomer.General properties
Appearance: | violet solid / solution |
Mass spec M+ increment: | 512.2 |
Molecular weight: | 512.56 |
CAS number: | 1192590-89-8 |
Molecular formula: | C28H28N6O4 |
Solubility: | Good in DMF, DMSO, alcohols, low solubility in water |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 541 |
ε, L⋅mol−1⋅cm−1: | 84000 |
Emission maximum, nm: | 567 |
Fluorescence quantum yield: | 0.1 |
CF260: | 0.32 |
CF280: | 0.19 |
Product citations
- Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
- Puthenveetil, R.; Lun, C.M.; Murphy, R.E.; Healy, L.B.; Vilmen, G.; Christenson, E.T.; Freed, E.O.; Banerjee, A. S-acylation of SARS-CoV-2 Spike Protein: Mechanistic Dissection, In Vitro Reconstitution and Role in Viral Infectivity. Journal of Biological Chemistry, 2021, 297(4), 101112. doi: 10.1016/j.jbc.2021.101112
- Chen, X.; Xu, J.; Wong, N.-K.; Zhong, S.; Yang, M.; Liu, Z.; Lu, Y.; Li, W.; Zhou, Y. Chemoproteomic profiling of cobalamin-independent methionine synthases in plant with a covalent probe. Journal of Agricultural and Food Chemistry, 2020, 68(30), 8050–8056. doi: 10.1021/acs.jafc.0c03301
- Xu, Y.; Deng, Z.; Shi, Y.; Chen, X.; Xu, J.; Zhong, S.; Xiao, Y.; Wong, N.-K.; Zhou, Y. Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe. Analytical Chemistry, 2020, 92(6), 4419–4426. doi: 10.1021/acs.analchem.9b05327
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