TAMRA azide, 6-isomer

Cat. # Quantity Price Lead time
A8130 1 mg $110 in stock
B8130 5 mg $135 in stock
C8130 10 mg $210 in stock
D8130 25 mg $390 in stock
E8130 50 mg $595 in stock
F8130 100 mg $790 in stock
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Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM and is sometimes used as a quencher of FAM.

Like other xanthenes, TAMRA exists as two isomers (5- and 6-) with very similar spectral properties. This is an azide derivative of the 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed click chemistry (CuAAC) or with cycloalkynes with copper-free strain-promoted alkyne azide cycloaddition (SPAAC) reaction.

Absorption and emission spectra of 6-TAMRA

Absorption and emission spectra of 6-TAMRA

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General properties

Appearance: violet solid / solution
Mass spec M+ increment: 512.2
Molecular weight: 512.56
CAS number: 1192590-89-8
Molecular formula: C28H28N6O4
Solubility: Good in DMF, DMSO, alcohols, low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Product citations

  1. Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
  2. Puthenveetil, R.; Lun, C.M.; Murphy, R.E.; Healy, L.B.; Vilmen, G.; Christenson, E.T.; Freed, E.O.; Banerjee, A. S-acylation of SARS-CoV-2 Spike Protein: Mechanistic Dissection, In Vitro Reconstitution and Role in Viral Infectivity. Journal of Biological Chemistry, 2021, 297(4), 101112. doi: 10.1016/j.jbc.2021.101112
  3. Chen, X.; Xu, J.; Wong, N.-K.; Zhong, S.; Yang, M.; Liu, Z.; Lu, Y.; Li, W.; Zhou, Y. Chemoproteomic profiling of cobalamin-independent methionine synthases in plant with a covalent probe. Journal of Agricultural and Food Chemistry, 2020, 68(30), 8050–8056. doi: 10.1021/acs.jafc.0c03301
  4. Xu, Y.; Deng, Z.; Shi, Y.; Chen, X.; Xu, J.; Zhong, S.; Xiao, Y.; Wong, N.-K.; Zhou, Y. Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe. Analytical Chemistry, 2020, 92(6), 4419–4426. doi: 10.1021/acs.analchem.9b05327
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