TAMRA azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A8130 | 1 mg | $110.00 | in stock | |
| B8130 | 5 mg | $180.00 | in stock | |
| C8130 | 10 mg | $310.00 | in stock | |
| D8130 | 25 mg |
$410.00
|
in stock | |
| E8130 | 50 mg |
$695.00
|
in stock | |
| F8130 | 100 mg |
$1190.00
|
in stock |
Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM and is sometimes used as a quencher of FAM.
Like other xanthenes, TAMRA exists as two isomers (5- and 6-) with very similar spectral properties. This is an azide derivative of the 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed click chemistry (CuAAC) or with cycloalkynes with copper-free strain-promoted alkyne azide cycloaddition (SPAAC) reaction.
Absorption and emission spectra of 6-TAMRA
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Water soluble thiol-reactive derivative of sulfo-Cyanine3, a Cy3 analog.THPTA ligand
THPTA is a water-soluble ligand for Cu(I) catalyzed click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.General properties
| Appearance: | violet solid / solution |
| Mass spec M+ increment: | 512.2 |
| Molecular weight: | 512.56 |
| CAS number: | 1192590-89-8 |
| Molecular formula: | C28H28N6O4 |
| Solubility: | Good in DMF, DMSO, alcohols, low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Product citations
- Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
- Puthenveetil, R.; Lun, C.M.; Murphy, R.E.; Healy, L.B.; Vilmen, G.; Christenson, E.T.; Freed, E.O.; Banerjee, A. S-acylation of SARS-CoV-2 Spike Protein: Mechanistic Dissection, In Vitro Reconstitution and Role in Viral Infectivity. Journal of Biological Chemistry, 2021, 297(4), 101112. doi: 10.1016/j.jbc.2021.101112
- Chen, X.; Xu, J.; Wong, N.-K.; Zhong, S.; Yang, M.; Liu, Z.; Lu, Y.; Li, W.; Zhou, Y. Chemoproteomic profiling of cobalamin-independent methionine synthases in plant with a covalent probe. Journal of Agricultural and Food Chemistry, 2020, 68(30), 8050–8056. doi: 10.1021/acs.jafc.0c03301
- Xu, Y.; Deng, Z.; Shi, Y.; Chen, X.; Xu, J.; Zhong, S.; Xiao, Y.; Wong, N.-K.; Zhou, Y. Molecular Imaging and In Situ Quantitative Profiling of Fatty Acid Synthase with a Chemical Probe. Analytical Chemistry, 2020, 92(6), 4419–4426. doi: 10.1021/acs.analchem.9b05327
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