Universal CPG type II, 1000A

Cat. # Quantity Price Lead time
2923-1g 1 g $45.00 10 days
2923-10g 10 g $410.00 10 days

The Universal CPG type II, 1000A is one of universal supports used to immobilize nucleosides for synthesizing oligonucleotides and to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking.

For the cleavage from the support and oligonucleotide deprotection anhydrous ammonia gas-phase, ammonium hydroxide/methylamine mixture and other basic reagents can be used in a short time. The Universal CPG type II, 1000A is suitable for use in harsh conditions and makes cleavage and deprotection faster compared to universal supports. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide - 40% methylamine (1:1).

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