Phosphate CPG 1000 solid support
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16860 | 100 mg | $40.00 | in stock | |
| 46860 | 1 g | $170.00 | in stock |
Phosphate modified oligos are used for gene synthesis by ligation, and other applications. Phosphate CPG 1000 is a modifier solid support for the synthesis of oligonucleotides bearing phosphate at 3 prime end.
CPG 1000 is suitable for the synthesis of long oligos up to 120 mer, the use of long oligos allows for a more efficient gene assembly. The CPG is compatible with standard synthesis and deblocking procedures, it ensures smooth cleavage and complete phosphorylation.
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This universal support is intended for automated synthesis of oligonucleotides to accelerate the dephosphorylation process during deblocking. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.General properties
| Appearance: | off-white beads |
| Quality control: | NMR 1H, HPLC-MS (95%) of bound reagent, loading measurement, functional testing (oligo synthesis) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30−50 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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