FAM DBCO, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151F0 | 1 mg | $110.00 | in stock | |
| 251F0 | 5 mg | $160.00 | in stock | |
| 451F0 | 25 mg | $210.00 | in stock | |
| 551F0 | 50 mg | $270.00 | in stock | |
| 651F0 | 100 mg |
$420.00
|
in stock |
Fluorescein (FAM) is one of the oldest yet well known fluorescent dyes. This derivative of FAM contains cyclooctyne group (dibenzocyclooctyne, abbreviated as DBCO or ADIBO) for the conjugation with azides via copper-free, strain-promoted azide alkyne cycloaddition (spAAc).
FAM DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. Azides react with DBCO spontaneously upon simple mixing. The reaction does not require any catalysts, and it is very efficient.
Fluorescein can be detected using a variety of different instruments.
Absorption and emission spectra of FAM
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BDP FL DBCO
BDP FL DBCO is a dye cyclooctyne for copper free Click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.FAM azide, 6-isomer
FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.General properties
| Appearance: | orange solid |
| Molecular weight: | 676.71 |
| Molecular formula: | C42H32N2O7 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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