FAM DBCO, 6-isomer

Cat. # Quantity Price Lead time
151F0 1 mg $110.00 5 days
251F0 5 mg $160.00 in stock
451F0 25 mg $210.00 in stock
551F0 50 mg $270.00 in stock
651F0 100 mg $420.00 in stock

Fluorescein (FAM) is one of the oldest yet well known fluorescent dyes. This derivative of FAM contains cyclooctyne group (dibenzocyclooctyne, abbreviated as DBCO or ADIBO) for the conjugation with azides via copper-free, strain-promoted azide alkyne cycloaddition (spAAc).

FAM DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. Azides react with DBCO spontaneously upon simple mixing. The reaction does not require any catalysts, and it is very efficient.

Fluorescein can be detected using a variety of different instruments.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: orange solid
Molecular weight: 676.71
Molecular formula: C42H32N2O7
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17
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