FAM DBCO, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151F0 | 1 mg | $110.00 | 5 days | |
| 251F0 | 5 mg | $160.00 | in stock | |
| 451F0 | 25 mg | $210.00 | in stock | |
| 551F0 | 50 mg | $270.00 | in stock | |
| 651F0 | 100 mg |
$420.00
|
in stock |
Fluorescein (FAM) is one of the oldest yet well known fluorescent dyes. This derivative of FAM contains cyclooctyne group (dibenzocyclooctyne, abbreviated as DBCO or ADIBO) for the conjugation with azides via copper-free, strain-promoted azide alkyne cycloaddition (spAAc).
FAM DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. Azides react with DBCO spontaneously upon simple mixing. The reaction does not require any catalysts, and it is very efficient.
Fluorescein can be detected using a variety of different instruments.
Absorption and emission spectra of FAM
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General properties
| Appearance: | orange solid |
| Molecular weight: | 676.71 |
| Molecular formula: | C42H32N2O7 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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