Cyanine3 hydrazide

Cyanine3 Hydrazides Microscopy
Cat. # Quantity Price Lead time
11070 1 mg $110.00 5 days
21070 5 mg $210.00 in stock
41070 25 mg $410.00 5 days
51070 50 mg $695.00 5 days
61070 100 mg $1190.00 in stock
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Cyanine3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide.

This reagent allows to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins which have undergone oxidative stress or deamination, or reducing saccarides. Cyanine3 is compatible with a number of fluorescent instruments.

Cyanine3 hydrazide structure
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Cyanine3 absorbance and emission spectra

Cyanine3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 543.57
Molecular formula: C30H40Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Zhang, J.; Chetnani, B.; Cormack, E.D.; Alonso, D.; Liu, W.; Mondragon, A.; Fei, J. Specific structural elements of the T-box riboswitch drive the two-step binding of the tRNA ligand. eLife, 2018, 7, e39518. doi: 10.7554/eLife.39518
  2. Cui, Z.; Wu, Y.; Mureev, S.; Alexandrov, K. Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro. Nucleic Acids Research, 2018, 46(12), 6387–6400. doi: 10.1093/nar/gky365
  3. Porciani, D.; Cardwell, L.N.; Tawiah, K.d.; Alam, K.K.; Lange, M.J.; Daniels, M.A.; Burke, D.H. Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines. Nature Communications, 2018, 9(1), 2283. doi: 10.1038/s41467-018-04691-x
  4. Poudyal, R.R.; Nguyen, P.D.M.; Lokugamage, M.P.; Callaway, M.K.; Gavette, J.V.; Krishnamurthy, R.; Burke, D.H. Nucleobase modification by an RNA enzyme. Nucleic Acids Research, 2017, 45(3), 1345–1354. doi: 10.1093/nar/gkw1199
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