AF 343 (Coumarin)-X-azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12630 | 1 mg | $105 | in stock | |
| 22630 | 5 mg | $165 | in stock | |
| 42630 | 25 mg | $285 | in stock | |
| 52630 | 50 mg |
$495
|
in stock | |
| 62630 | 100 mg |
$880
|
in stock |
AF 343 (Coumarin) is a blue emitting fluorophore with an emission maximum of around 480 nm. This dye forms a FRET pair with fluorescein and can harvest blue light energy for the subsequent transfer to other fluorophores.
The azide derivative can be conjugated with alkynes in copper-catalyzed and copper-free сlick reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye and the azide function.
Absorption and emission spectra of AF 343 (Coumarin)
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AF 343 (Coumarin)-X-NHS ester
AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.DBCO-NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.General properties
| Appearance: | yellow solid |
| Mass spec M+ increment: | 480.3 |
| Molecular weight: | 480.56 |
| Molecular formula: | C25H32N6O4 |
| IUPAC name: | 5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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