Coumarin 343 X azide

Cat. # Quantity Price Lead time
12630 1 mg $110.00 in stock
22630 5 mg $210.00 in stock
42630 25 mg $410.00 in stock
52630 50 mg $695.00 in stock
62630 100 mg $1190.00 in stock

Coumarin 343 is a blue emitting fluorophore with an emission maximum around 480 nm. This dye forms a FRET pair with fluorescein, and can harvest blue light energy for the subsequent transfer to other fluorophores.

The azide derivative can be conjugated with alkynes in a copper-catalyzed and copper-free Click chemistry reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye, and the azide function.

Absorption and emission spectra of Coumarin 343

Absorption and emission spectra of Coumarin 343

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General properties

Appearance: yellow solid
Mass spec M+ increment: 480.3
Molecular weight: 480.56
Molecular formula: C25H32N6O4
IUPAC name: 5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24
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