BDP FL NHS ester

Cat. # Quantity Price Lead time
11420 1 mg $125 $98 in stock
21420 5 mg $260 $175 in stock
41420 25 mg $485 in stock
51420 50 mg $824 in stock
61420 100 mg $1380 in stock
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BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein. An amino-reactive dye for the labeling of proteins and peptides.

While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY™ FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.

The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.

The dye is a good replacement for fluorescein (FAM), DyLight™ 488, Cy2™, and other 488 nm dyes.

Absorption and emission spectra of BDP FL

Absorption and emission spectra of BDP FL

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General properties

Appearance: orange solid
Mass spec M+ increment: 274.1
Molecular weight: 389.16
CAS number: 146616-66-2
Molecular formula: C18H18BF2N3O4
Solubility: Good in organic solvents (DMF, DMSO, dichlromethane), limited in water
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 92000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

Product citations

  1. Tereshchenkov, A. G.; Khairullina, Z. Z.; Volynkina, I. A.; Lukianov, D. A.; Nazarov, P. A.; Pavlova, J. A.; Tashlitsky, V. N.; Razumova, E. A.; Ipatova, D. A.; Timchenko, Y. V.; Senko, D. A.; Efremenkova, O. V.; Paleskava, A.; Konevega, A. L.; Osterman, I. A.; Rodin, I. A.; Sergiev, P. V.; Dontsova, O. A.; Bogdanov, A. A.; Sumbatyan, N. V. Triphenylphosphonium Analogs of Short Peptide Related to Bactenecin 7 and Oncocin 112 as Antimicrobial Agents. Pharmaceutics, 2024, 16(1), 148. doi: 10.3390/pharmaceutics16010148
  2. Kicková, E.; Sadeghi, A.; Puranen, J.; Tavakoli, S.; Sen, M.; Ranta, V.-P.; Arango-Gonzalez, B.; Bolz, S.; Ueffing, M.; Salmaso, S.; Caliceti, P.; Toropainen, E.; Ruponen, M.; Urtti, A. Pharmacokinetics of Pullulan–Dexamethasone Conjugates in Retinal Drug Delivery. Pharmaceutics, 2023, 14(1), 12. doi: 10.3390/pharmaceutics14010012
  3. Ji, F.; Hur, M.; Hur, S.; Wang, S.; Sarkar, P.; Shao, S.; Aispuro, D.; Cong, X.; Hu, Y.; Li, Z.; Xue, M. Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage. ACS Bio Med Chem Au, 2023, 3(3), 283–294. doi: 10.1021/acsbiomedchemau.3c00018
  4. Daumar, P.; Goisnard, A.; Dubois, C.; Roux, M.; Depresle, M.; Penault-Llorca, F.; Bamdad, M.; Mounetou, E. Chemical biology fluorescent tools for in vitro investigation of the multidrug resistant P-glycoprotein (P-gp) expression in tumor cells. RSC Advances, 2023, 13(39), 27016-27035. doi: 10.1039/d3ra05093a
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