BDP® FL NHS ester
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
11420 | 1 mg | $125 $98 | in stock | |
21420 | 5 mg | $260 $175 | in stock | |
41420 | 25 mg | $485 | in stock | |
51420 | 50 mg | $824 | in stock | |
61420 | 100 mg | $1380 | in stock |
BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein. An amino-reactive dye for the labeling of proteins and peptides.
While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BDP FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.
The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.
The dye is a good replacement for fluorescein (FAM), DyLight™ 488, Cy2™, and other 488 nm dyes.
Absorption and emission spectra of BDP FL
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Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.Cyanine3 amine
Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.General properties
Appearance: | orange solid |
Mass spec M+ increment: | 274.1 |
Molecular weight: | 389.16 |
CAS number: | 146616-66-2 |
Molecular formula: | C18H18BF2N3O4 |
Solubility: | Good in organic solvents (DMF, DMSO, dichlromethane), limited in water |
Quality control: | NMR 1H, HPLC-MS (95+%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 503 |
ε, L⋅mol−1⋅cm−1: | 92000 |
Emission maximum, nm: | 509 |
Fluorescence quantum yield: | 0.97 |
CF260: | 0.015 |
CF280: | 0.027 |
Product citations
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- Kicková, E.; Sadeghi, A.; Puranen, J.; Tavakoli, S.; Sen, M.; Ranta, V.-P.; Arango-Gonzalez, B.; Bolz, S.; Ueffing, M.; Salmaso, S.; Caliceti, P.; Toropainen, E.; Ruponen, M.; Urtti, A. Pharmacokinetics of Pullulan–Dexamethasone Conjugates in Retinal Drug Delivery. Pharmaceutics, 2023, 14(1), 12. doi: 10.3390/pharmaceutics14010012
- Ji, F.; Hur, M.; Hur, S.; Wang, S.; Sarkar, P.; Shao, S.; Aispuro, D.; Cong, X.; Hu, Y.; Li, Z.; Xue, M. Multiplex Protein Imaging through PACIFIC: Photoactive Immunofluorescence with Iterative Cleavage. ACS Bio Med Chem Au, 2023, 3(3), 283–294. doi: 10.1021/acsbiomedchemau.3c00018
- Daumar, P.; Goisnard, A.; Dubois, C.; Roux, M.; Depresle, M.; Penault-Llorca, F.; Bamdad, M.; Mounetou, E. Chemical biology fluorescent tools for in vitro investigation of the multidrug resistant P-glycoprotein (P-gp) expression in tumor cells. RSC Advances, 2023, 13(39), 27016-27035. doi: 10.1039/d3ra05093a