AMCA NHS ester

Cat. # Quantity Price Lead time
3606-5mg 5 mg $110 in stock
3606-25mg 25 mg $290 in stock
3606-50mg 50 mg $435 in stock
3606-100mg 100 mg $595 in stock
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Activated NHS-ester of AMCA (aminomethyl coumarin acetate) dye. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

AMCA is one of the brightest blue fluorescent dyes. This fluorophore has a relatively large Stoke’s shift, high resistance to photobleaching, and pH-independent fluorescence from pH 4 to 10. AMCA is a widely used fluorophore for multiple-color labeling due to its minimal fluorescence overlap with green- and longer wavelength-emitting fluorescent dyes.

Absorption and emission spectra of AMCA

Absorption and emission spectra of AMCA

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General properties

Appearance: gray powder
Mass spec M+ increment: 331.3
Molecular weight: 366.76
CAS number: 113721-87-2
Molecular formula: C16H15ClN2O6
Solubility: in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 348
ε, L⋅mol−1⋅cm−1: 17400
Emission maximum, nm: 435
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.13
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