Cyanine5 carboxylic acid

Cat. # Quantity Price Lead time
13090 1 mg $110.00 5 days
23090 5 mg $210.00 in stock
43090 25 mg $410.00 in stock
53090 50 mg $695.00 in stock
63090 100 mg $1190.00 in stock

Non-activated carboxylic acid, an analog of Cy5® free carboxylic acid. Contains Cyanine5 fluorophore.

This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. Water-soluble version is available.

This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration. For coupling with amines and protein labeling, consider using Cyanine5 NHS ester, or water-soluble sulfo-Cyanine5 NHS ester.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 519.12
CAS number: 1032678-07-1 (chloride), 195867-59-5 (inner salt), 766503-38-2 (without anion)
Molecular formula: C32H39ClN2O2
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-​, chloride
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Santra, K.; Clark, K.D.; Maity, N.; Petrich, J.W.; Anderson, J.L. Exploiting Fluorescence Spectroscopy to Identify Magnetic Ionic Liquids Suitable for the Isolation of Oligonucleotides. The Journal of Physical Chemistry B, 2018, 122(31), 7747–7756. doi: 10.1021/acs.jpcb.8b05580
  2. Semkina, A.S.; Abakumov, M.A.; Skorikov, A.S.; Abakumova, T.O.; Melnikov, P.A.; Grinenko, N.F.; Cherepanov, S.A.; Vishnevskiy, D.A.; Naumenko, V.A.; Ionova, K.P.; Majouga, A.G.; Chekhonin, V.P. Multimodal doxorubicin loaded magnetic nanoparticles for {VEGF} targeted theranostics of breast cancer. Nanomedicine: Nanotechnology, Biology and Medicine, 2018, 14(5), 1733–1742. doi: 10.1016/j.nano.2018.04.019
  3. Kwok, S.J.J.; Choi, M.; Bhayana, B.; Zhang, X.; Ran, C.; Yun, S.-H. Two-photon excited photoconversion of cyanine-based dyes. Scientific Reports, 2016, 6, 23866. doi: 10.1038/srep23866
  4. Gupta, A.; Verma, N.C.; Khan, S.; Tiwari, S.; Chaudhary, A.; Nandi, C.K. Paper strip based and live cell ultrasensitive lead sensor using carbon dots synthesized from biological media. Sensors and Actuators B: Chemical, 2016, 232, 107–114. doi: 10.1016/j.snb.2016.03.110
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