Cyanine5 carboxylic acid

Cat. # Quantity Price Lead time
13090 1 mg $110 in stock
23090 5 mg $210 in stock
43090 25 mg $410 in stock
53090 50 mg $695 in stock
63090 100 mg $1190 in stock
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Non-activated carboxylic acid, an analog of Cy5® free carboxylic acid. Contains Cyanine5 fluorophore.

This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. Water-soluble version is available.

This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration. For coupling with amines and protein labeling, consider using Cyanine5 NHS ester, or water-soluble sulfo-Cyanine5 NHS ester.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark golden solid
Molecular weight: 519.12
CAS number: 1032678-07-1 (chloride), 195867-59-5 (inner salt), 766503-38-2 (without anion)
Molecular formula: C32H39ClN2O2
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-​, chloride
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), very poorly soluble in water (0.25 mM, 130 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Tchatchiashvilli, T.; Duering, H.; Mueller-Boetticher, L.; Grune, C.; Fischer, D.; Pletz, M.W.; Makarewicz, O. PEG-PLGA nanoparticles deposited in Pseudomonas aeruginosa and Burkolderia cenocepacia. Journal of Pharmaceutical Analysis, 2024. doi: 10.1016/j.jpha.2024.01.007
  2. Yao, P.; Wang, X.; Wang, Q.; Dai, Q.; Peng, Y.; Yuan, Q.; Mou, N.; Lv, S.; Weng, B.; Wang, Y.; Sun, F. Cyclic RGD-Functionalized PH/ROS Dual-Responsive Nanoparticle for Targeted Breast Cancer Therapy. Pharmaceutics, 2023, 15(7), 1827. doi: 10.3390/pharmaceutics15071827
  3. Lu, Y.; Zhou, J.; Wang, Q.; Cai, J.; Yu, B.; Dai, Q.; Bao, Y.; Chen, R.; Zhang, Z.; Zhang, D.; Hou, T. Glucocorticoid-Loaded PH/ROS Dual-Responsive Nanoparticles Alleviate Joint Destruction by Downregulating the NF-ΚB Signaling Pathway. Acta Biomaterialia, 2023, 164, 458–473. doi: 10.1016/j.actbio.2023.04.012
  4. Rezaei, S.; de Araújo Júnior, R. F.; da Silva, I. L. G.; Schomann, T.; Eich, C.; Cruz, L. J. Erythrocyte−cancer Hybrid Membrane-Coated Reduction-Sensitive Nanoparticles for Enhancing Chemotherapy Efficacy in Breast Cancer. Biomaterials Advances, 2023, 151, 213456. doi: 10.1016/j.bioadv.2023.213456
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