FAM Phosphoramidite, 6-Isomer

Fluorescein (FAM) Oligonucleotide synthesis Phosphoramidite
Cat. # Quantity Price Lead time
C5160 100 umol (85 mg) $60.00 in stock
D5160 250 mg $90.00 5 days
F5160 1 g $190.00 in stock
G5160 5 g $930.00 21 days
H5160 10 g $1800.00 21 days
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Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

FAM Phosphoramidite, 6-Isomer
6-FAM phosphoramidite structure
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FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: white foam
Molecular weight: 843.94
CAS number: 204697-37-0
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM.
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

Product citations

  1. Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726
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