FAM phosphoramidite, 6-isomer

FAM (Fluorescein) Oligonucleotide synthesis Phosphoramidites
Cat. # Quantity Price Lead time
D5160
/ Easily soluble lyophilised form in 8 mL septum
250 mg $97 in stock
F5160
/ Easily soluble lyophilised form in 30 mL septum
1 g $190 $147 in stock
G5160 5 g $740 5 days
H5160 10 g $1290 $970 5 days
K5160 50 g please inquire in stock
L5160 100 g please inquire 21 days
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Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

FAM phosphoramidite, 6-isomer, F5160 / Easily soluble lyophilised form in 30 mL septum
6-FAM phosphoramidite structure
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FAM absorbance and emission spectra

FAM absorbance and emission spectra

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General properties

Appearance: off-white solid
Molecular weight: 843.94
CAS number: 204697-37-0
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

Product citations

  1. Zharkov, T.D.; Mironova, E.M.; Markov, O.V.; Zhukov, S.A.; Khodyreva, S.N.; Kupryushkin, M.S. Fork- and Comb-like Lipophilic Structures: Different Chemical Approaches to the Synthesis of Oligonucleotides with Multiple Dodecyl Residues. International Journal of Molecular Sciences, 2023, 24(19), 14637. doi: 10.3390/ijms241914637
  2. Bulgakova, A.; Chubarov, A.; Dmitrienko, E. Magnetic Nylon 6 Nanocomposites for the Microextraction of Nucleic Acids from Biological Samples. Magnetochemistry, 2022, 8(8), 85. doi: 10.3390/magnetochemistry8080085
  3. Stanton, J.-A.L.; O'Brien, R.; Hall, R.J.; Chernyavtseva, A.; Ha, H.J.; Jelley, L.; Mace, P.D.; Klenov, A.; Treece, J.M.; Fraser, J.D.; Clow, F.; Clarke, L.; Su, Y.; Kurup, H.M.; Filichev, V.V.; Rolleston, W.; Law, L.; Rendle, P.M.; Harris, L.D.; Wood, J.M.; Scully, T.W.; Ussher, J.E.; Grant, J.; Hore, T.A.; Moser, T.V.; Harfoot, R.; Lawley, B.; Quiñones-Mateu, M.E.; Collins, P.; Blaikie, R. Uncoupling Molecular Testing for SARS-CoV-2 From International Supply Chains. Frontiers in Public Health, 2022, 9, 808751. doi: 10.3389/fpubh.2021.808751
  4. Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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