FAM Phosphoramidite, 6-Isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| C5160 | 100 umol (85 mg) | $60.00 | 5 days | |
| D5160 | 250 mg | $90.00 | in stock | |
| F5160 | 1 g | $190.00 | 5 days | |
| G5160 | 5 g |
$930.00
|
5 days | |
| H5160 | 10 g |
$1800.00
|
21 days |
Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
FAM absorbance and emission spectra
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FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.General properties
| Appearance: | white foam |
| Molecular weight: | 843.94 |
| CAS number: | 204697-37-0 |
| Molecular formula: | C46H58N3O10P |
| Solubility: | Good solubility in acetonitrile and DCM. |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Oligo synthesis details
| Coupling conditions: | coupling time 10 min |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine |
Product citations
- Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726
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