AF 430 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg |
$110
|
in stock | |
| 20820 | 5 mg |
$165
|
in stock | |
| 40820 | 25 mg |
$380
|
in stock | |
| 50820 | 50 mg |
$695
|
in stock | |
| 60820 | 100 mg |
$1190
|
in stock |
AF 430 is a hydrophilic dye. It is used in flow cytometry and other applications.
The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.
Absorption and emission spectra of AF 430
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Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.General properties
| Appearance: | yellow powder |
| Molecular weight: | 701.75 |
| CAS number: | 467233-94-9 |
| Molecular formula: | C32H42N3F3O9S |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
| CF260: | 0,59 |
| CF280: | 0,24 |
Short link - lumiprobe.com/sh/p/6O
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