HMRhoNox-M, Fe(II)-selective fluorescent probe

Microscopy Flow cytometry
Cat. # Quantity Price Lead time
3317-50ug 50 ug $120 in stock
3317-500ug 500 ug $590 in stock
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HMRhoNox-M (also known as LysoRhoNox) is a Fe2+-selective fluorescent probe based on the N-oxide-controlled spirocyclization of tetramethyl-hydroxymethyl rhodamine.

In the absence of Fe2+, HMRhoNox-M exists in the non-fluorescent spirocyclic form showing only negligible fluorescence in an aqueous buffer and at physiological pH. The addition of Fe2+ induces a 60-fold increase of the fluorescence signal at 575 nm through the deoxygenation of the dialkylamino group and the transition of the probe to an open fluorescent form. HMRhoNox-M responds to Fe2+ in a dose-dependent manner.

The fluorescence response of HMRhoNox-M is highly selective for Fe2+ over other transition metal ions, including Fe3+, alkali metal ions, and alkaline earth metal ions.

HMRhoNox-M is the cell-permeant probe that is mainly localized in lysosomes. It is suitable for monitoring fluctuations of endogenous labile iron in living cells, including the transferrin-induced Fe uptake.

A549 cells stained with Fe(II)-selective fluorescent probe HMRhoNox-M (3317-x) and DAPI. Adding Fe(II) induces a 60-fold increase in the HMRhoNox-M fluorescence.
HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
HMRhoNox-M, Fe(II)-selective fluorescent probe, 3317-10mg
Structure of HMRhoNox-M
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Absorption and emission spectra of HMRhoNox-M

Absorption and emission spectra of HMRhoNox-M

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General properties

Appearance: beige-pinkish crystals
Molecular weight: 388.47
Molecular formula: C24H24N2O3
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
Emission maximum, nm: 575
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