AMCA streptavidin

Cat. # Quantity Price Lead time
A1AS0 100 ug –   20 days
11AS0 1 mg $165 20 days
Found better price? Let us know and we will propose the way forward!

Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.

Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.

This streptavidin is a lyophilized conjugate with AMCA, one of the brightest blue fluorescent dyes.

The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.

Absorption and emission spectra of AMCA

Absorption and emission spectra of AMCA

Customers also purchased with this product

Biotin-cystamine hydrochloride

Biotin and cystamine conjugate is a cleavable biotinylation reagent.

Biotin-PEG4-NHS ester

PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.


Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe to streptavidin.

General properties

Appearance: white solid
Solubility: good in water
Quality control: Functional test, gel electrophoresis
Storage conditions: Transportation: at room temperature for 1 week. Store at -20°С 9 months.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 348
ε, L⋅mol−1⋅cm−1: 17400
Emission maximum, nm: 435
Fluorescence quantum yield: 0.91
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.