PreciseGreen® dsDNA Quantification Reagent, 200×

Nucleic acid quantification PCR and qPCR Fluorometry
Cat. # Quantity Price Lead time
12010 100 uL $46 in stock
42010 1 mL $360 in stock
52010 10 mL $2350 in stock
62010 25 mL please inquire in stock
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PreciseGreen® is a DNA-binding benzothiazole dye. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double-stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and dedicated DNA measurement fluorometers.

The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit, including quantification dye, is also available.

PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen dsDNA quantification reagent, 200x solution in DMSO, 42010
PreciseGreen fluorescence emission vs DNA concentration
Fluorescence of the PreciseGreen Quantitation Reagent upon binding with dsDNA, ssDNA and RNA
Structure of PreciseGreen
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PreciseGreen excitation and emission spectra

PreciseGreen excitation and emission spectra

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General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
Emission maximum, nm: 526

Product citations

  1. Vellani, V.; Cuccaro, A.; Oliva, M.; Pretti, C.; Renzi, M. Assessing combined effects of long-term exposure to copper and marine heatwaves on the reef-forming serpulid Ficopomatus enigmaticus through a biomarker approach. Marine Pollution Bulletin, 2024, 201, 116269. doi: 10.1016/j.marpolbul.2024.116269
  2. Stamm, S.M.; Wagner, R.; Lang, D.A.; Skerra, A.; Gebauer, M. Development of a Clonal and High-Yield Mammalian Cell Line for the Manufacturing of a Hyperactive Human DNase I with Extended Plasma Half-Life Using PASylation ® Technology. Pharmaceutics, 2024, 16(7), 967. doi: 10.3390/pharmaceutics16070967
  3. Hernández-Rollán, C.; Ehrmann, A.K.; Vlassis, A.; Kandasamy, V.; Nørholm, M.H.H. Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR. BMC Biotechnology, 2024, 24, 17. doi: 10.1186/s12896-024-00844-7
  4. Kang, Z.; Fu, P.; Ma, H.; Li, T.; Lu, K.; Liu, J.; Ginjala, V.; Romanienko, P.; Feng, Z.; Guan, M.; Ganesan, S.; Xia, B. Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy. bioRxiv, 2023, preprint. doi: 10.1101/2023.10.03.560719
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This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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