ROX reference dye for qPCR

Cat. # Quantity Price Lead time
31110 500 uL –   in stock
41110 1 mL $35 in stock
51110 25 mL $265 in stock
61110 50 mL $345 in stock
Found better price? Let us know and we will propose the way forward!

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.

The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

Customers also purchased with this product

TAMRA alkyne, 5-isomer

Alkyne derivative of TAMRA dye, pure 5-isomer for сopper-catalyzed сlick chemistry.

Biotin-11-dUTP

Biotin-labeled deoxyuridine triphosphate for enzymatic incorporation into DNA and indirect detection.

JOE alkyne, 6-isomer

JOE dye alkyne derivative for copper-catalyzed click chemistry reaction with azides. JOE dye is widely used for nucleic acid labeling.

General properties

Appearance: pink solution
Quality control: functional test in RT PCR, UV-Vis abs
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
Emission maximum, nm: 591

Product citations

  1. Bartlett, C.; Langsjoen, J.; Cheng, Q.; Yingling, A. V.; Weiss, M.; Bradfute, S.; Perkins, D. J.; Hurwitz, I. COVID-19 Global Pandemic Planning: Presence of SARS-CoV-2 Fomites in a University Hospital Setting. Exp Biol Med (Maywood), 2021, 246(18), 2039–2045. doi: 10.1177/15353702211024597
  2. Zhuo, X.-Z.; Bai, K.; Wang, Y.; Liu, P.; Xi, W.; She, J.; Liu, J. Long-chain non-coding RNA-GAS5 / hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2. Bioscience Reports, 2021, 41(9), BSR20202232. doi: 10.1042/BSR20202232
  3. Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.