ROX reference dye for qPCR

Cat. # Quantity Price Lead time
31110 500 uL –   in stock
41110 1 mL $35 in stock
51110 25 mL $265 in stock
61110 50 mL $345 in stock
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This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows for correcting pipetting errors and instrumental drift, such as changes in lamp intensity output over time.

The dye contains 25 μM solution of rhodamine 101 reference dye in 10 mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

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General properties

Appearance: pink solution
Quality control: functional test in RT PCR, UV-Vis abs
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
Emission maximum, nm: 591

Product citations

  1. Bartlett, C.; Langsjoen, J.; Cheng, Q.; Yingling, A. V.; Weiss, M.; Bradfute, S.; Perkins, D. J.; Hurwitz, I. COVID-19 Global Pandemic Planning: Presence of SARS-CoV-2 Fomites in a University Hospital Setting. Exp Biol Med (Maywood), 2021, 246(18), 2039–2045. doi: 10.1177/15353702211024597
  2. Zhuo, X.-Z.; Bai, K.; Wang, Y.; Liu, P.; Xi, W.; She, J.; Liu, J. Long-chain non-coding RNA-GAS5 / hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2. Bioscience Reports, 2021, 41(9), BSR20202232. doi: 10.1042/BSR20202232
  3. Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
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