ROX reference dye for qPCR
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
31110 | 500 uL | – | in stock | |
41110 | 1 mL | $35 | in stock | |
51110 | 25 mL | $265 | in stock | |
61110 | 50 mL | $345 | in stock |
This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows for correcting pipetting errors and instrumental drift, such as changes in lamp intensity output over time.
The dye contains 25 μM solution of rhodamine 101 reference dye in 10 mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.
ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.
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dsGreen is a stain with high sensitivity and selectivity for dsDNA. This product is a solid form of dsGreen that can be used for the preparation of solutions.General properties
Appearance: | pink solution |
Quality control: | functional test in RT PCR, UV-Vis abs |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 570 |
Emission maximum, nm: | 591 |
Product citations
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- Zhuo, X.-Z.; Bai, K.; Wang, Y.; Liu, P.; Xi, W.; She, J.; Liu, J. Long-chain non-coding RNA-GAS5 / hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2. Bioscience Reports, 2021, 41(9), BSR20202232. doi: 10.1042/BSR20202232
- Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038