sulfo-Cyanine5 bis-NHS ester

NHS esters sulfo-Cyanine5

Cat. #	Quantity	Price	Lead time
133D0	1 mg	$125	in stock
233D0	5 mg	$325	in stock
433D0	25 mg	$850	in stock
533D0	50 mg	$1490	in stock
633D0	100 mg	$1990	in stock

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sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5® bis-NHS ester.

The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.

Structure of Sulfo-Cyanine5 bis-NHS ester

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Absorption and emission spectra of sulfo-Cyanine5

General properties

Appearance:	dark blue powder
Molecular weight:	975.14
Molecular formula:	C₄₅H₅₁KN₄O₁₄S₂
Solubility:	good in DMF, DMSO, water
Quality control:	NMR ¹H, HPLC-MS (895%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	271000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.28
CF₂₆₀:	0.04
CF₂₈₀:	0.04

Product citations

Michalski, J.; Kalwarczyk, T.; Kwapiszewska, K.; Enderlein, J.; Poniewierski, A.; Karpińska, A.; Kucharska, K.; Hołyst, R. Rotational and translational diffusion of biomolecules in complex liquids and HeLa cells. Soft Matter, 2024, 20, 5810-5821. doi: 10.1039/d4sm00422a
Pettersson, J.R.; Lanni, F.; Rule, G.S. Dual lifetimes for complexes between Glutathione-S-transferase (hGSTA1-1) and product-like ligands detected by single-molecule fluorescence imaging. Biochemistry, 2017, 56(31), 4073–4083. doi: 10.1021/acs.biochem.7b00030

Cy® is a trademark of GE Healthcare.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.