Sulfo-Cyanine5 bis-NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133D0 | 1 mg | $110.00 | in stock | |
| 233D0 | 5 mg | $290.00 | in stock | |
| 433D0 | 25 mg |
$690.00
|
in stock | |
| 533D0 | 50 mg |
$1270.00
|
in stock | |
| 633D0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine5 bis-NHS ester is a water soluble bifunctional dye possessing two NHS ester functions. It is an analog of Cy5® bis-NHS ester.
The dye is useful for the cross-conjugation of amines, and for other advanced labeling applications.
Absorption and emission spectra of sulfo-Cyanine5
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Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.Coumarin 343 X azide
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.FAM azide, 5-isomer
FAM (fluorescein) azide for Click chemistry, pure 5-isomer.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 975.14 |
| Molecular formula: | C45H51KN4O14S2 |
| Solubility: | good in DMF, DMSO, water |
| Quality control: | NMR 1H, HPLC-MS (895%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Pettersson, J.R.; Lanni, F.; Rule, G.S. Dual lifetimes for complexes between Glutathione-S-transferase (hGSTA1-1) and product-like ligands detected by single-molecule fluorescence imaging. Biochemistry, 2017, 56(31), 4073–4083. doi: 10.1021/acs.biochem.7b00030
Cy® is a trademark of GE Healthcare.
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