Pico488 dsDNA Quantification Kit

PCR and qPCR Nucleic acid quantification
Cat. # Quantity Price Lead time
1102-20
20 assays
–   1 days
1102-200
/ 1 mL dye
200 assays
$450.00 1 days
B1102
/ 10 x 100 uL dye
200 assays
$500.00 1 days
Sample request

Kit for quantitative measurement of double-stranded DNA in samples in the presence of RNA, proteins, and common contaminants. Pico488 allows to quantitate as little as low pg/mL of dsDNA. The range of linearity for DNA concentration measurements
is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS).

The kit contains Pico488 dye, buffer, and DNA standard for the measurements. It is compatible with fluorometers, fluorescent plate readers, cuvette and microvolume fluorometers.

Pico488 dsDNA quantification kit, 1102-200
Pico488 dsDNA quantification kit, 1102-200
Fluorescence of the Pico488 Quantitation Reagent upon binding with dsDNA, ssDNA and RNA
Pico488 fluorescence emission vs DNA concentration
Excitation and emission spectra of Pico488 complex with DNA

Ready-to-use kit manual

Calculator

General properties

Storage conditions: Store at temperature below 4°С. Do not freeze!
MSDS: Download

Kit

Storage time in months: 12

References

  1. Izzo, A.; Akol, I.; Villarreal, A.; Garcia-Miralles, M.; Bovio, P.; Heidrich, S.; Vogel, T. Nucleophosmin 1 Cooperates with the Methyltransferase DOT1L to Regulate H3K79me2 Levels and DNA Satellites Expression at Peri-Nucleolar Heterochromatin. Research Square, 2023, preprint. doi: 10.21203/rs.3.rs-2745386/v1
  2. Psotta, C.; Chaturvedi, V.; Gonzalez-Martinez, J. F.; Sotres, J.; Falk, M. Portable Prussian Blue-Based Sensor for Bacterial Detection in Urine. Sensors, 2023, 23(1), 388. doi: 10.3390/s23010388
  3. Sharma, A. K.; Fitieh, A. M.; Locke, A. J.; Ali, J. Y. H.; Ismail, I. H. Quantification of Protein Enrichment at Site-Specific DNA Double-Strand Breaks by Chromatin Immunoprecipitation in Cultured Human Cells. STAR Protocols, 2023, 4(1), 101917. doi: 10.1016/j.xpro.2022.101917
  4. Monzó, C.; Gkioni, L.; Beyer, A.; Valenzano, D. R.; Grönke, S.; Partridge, L. Dietary Restriction Mitigates the Age-Associated Decline in Mouse B Cell Receptor Repertoire Diversity. Cell Reports, 2023, 42(7), 112722. doi: 10.1016/j.celrep.2023.112722
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