sulfo-Cyanine3.5 azide

Cat. # Quantity Price Lead time
A2330 1 mg $125 in stock
B2330 5 mg $325 in stock
C2330 10 mg $470 in stock
D2330 25 mg $850 in stock
E2330 50 mg $1490 in stock
F2330 100 mg $1990 in stock
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sulfo-Cyanine3.5 is a fluorescent dye with absorption and emission spectra between those of Cyanine3 and Cyanine5. Because of its spectral characteristics, this fluorophore is widely used in BRET and FRET experiments both as a pair donor and acceptor.

sulfo-Cyanine3.5 is a sulfonated derivative of Cyanine3.5 dye because it contains four additional sulfo groups, which ensures high water solubility of the dye and its conjugates. This allows working with this reagent in aqueous solutions without adding organic solvents.

This reagent is an azide derivative of sulfo-Cyanine3.5 dye for click chemistry reactions. The azide group allows conjugating the fluorophore with molecules containing alkyne or cycloalkyne groups, including various biomolecules, small molecules, and polymers, using copper-catalyzed click chemistry and copper-free SPAAC reaction.

Absorption and emission spectra of sulfo-Cyanine3.5

Absorption and emission spectra of sulfo-Cyanine3.5

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General properties

Appearance: dark colored solid
Mass spec M+ increment: 959.2
Molecular weight: 1073.37
Molecular formula: C41H43N6K3O13S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17
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