Cyanine3 alkyne

Cat. # Quantity Price Lead time
A10B0 1 mg $125 in stock
B10B0 5 mg $260 in stock
C10B0 10 mg $325 in stock
D10B0 25 mg $510 in stock
E10B0 50 mg $895 in stock
F10B0 100 mg $1490 in stock
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Cyanine3 alkyne is a bright and photostable fluorophore for сlick сhemistry, an analog of Cy3®. This dye can be attached to nearly any molecule bearing azide group via copper-catalyzed click-reaction.

This alkyne is non-water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins or peptides.

Cyanine3 absorbance and emission spectra

Cyanine3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 530.14
Molecular formula: C33H40ClN3O
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), , practically insoluble in water (50 mg/L = 80 uM)
Quality control: NMR 1H (95%), HPLC-MS
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Scientific Reports, 2021, 11(1), 12229. doi: 10.1038/s41598-021-91612-6.
  2. Guarin, M.; Ny, A.; De Croze, N.; Maes, J.; Léonard, M.; Annaert, P.; de Witte, P. A. M. Pharmacokinetics in Zebrafish Embryos (ZFE) Following Immersion and Intrayolk Administration: A Fluorescence-Based Analysis. Pharmaceuticals, 2021, 14(6), 576. doi: 10.3390/ph14060576
  3. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal imaging and pharmacokinetics of fluorescent compounds in zebrafish eleuthero-embryos after different routes of administration. Scientific Reports, 2021, 11, 12229. doi: 10.1038/s41598-021-91612-6
  4. Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
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