Cyanine3 alkyne

Cat. # Quantity Price Lead time
A10B0 1 mg $110.00 in stock
B10B0 5 mg $210.00 in stock
C10B0 10 mg $310.00 in stock
D10B0 25 mg $410.00 in stock
E10B0 50 mg $695.00 in stock
F10B0 100 mg $1190.00 in stock

Cyanine3 alkyne is a fluorophore ready for the use in Click Chemistry reaction.

With this reagent, bright and photostable Cyanine3 reporter, an analog of Cy3®, can be attached to nearly any molecule bearing azide group.

This alkyne is non water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins, or peptides.

Cyanine3 absorbance and emission spectra

Cyanine3 absorbance and emission spectra

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General properties

Appearance: red powder
Molecular weight: 530.14
Molecular formula: C33H40ClN3O
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), , practically insoluble in water (50 mg/L = 80 uM)
Quality control: NMR 1H (95%), HPLC-MS
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
  2. Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, 2017, 292(11), 4499–4518. doi: 10.1074/jbc.M116.726752
  3. Ponomarenko, A.I.; Brylev, V.A.; Sapozhnikova, K.A.; Ustinov, A.V.; Prokhorenko, I.A.; Zatsepin, T.S.; Korshun, V.A. Tetrahedral DNA conjugates from pentaerythritol-based polyazides. Tetrahedron, 2016, 72(19), 2386–2391. doi: 10.1016/j.tet.2016.03.051
  4. Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
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