Cyanine3 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A10B0 | 1 mg | $110.00 | in stock | |
| B10B0 | 5 mg | $210.00 | in stock | |
| C10B0 | 10 mg | $310.00 | in stock | |
| D10B0 | 25 mg |
$410.00
|
in stock | |
| E10B0 | 50 mg |
$695.00
|
in stock | |
| F10B0 | 100 mg |
$1190.00
|
in stock |
Cyanine3 alkyne is a fluorophore ready for the use in Click Chemistry reaction.
With this reagent, bright and photostable Cyanine3 reporter, an analog of Cy3®, can be attached to nearly any molecule bearing azide group.
This alkyne is non water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins, or peptides.
Cyanine3 absorbance and emission spectra
Recommended protocol
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FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.TAMRA alkyne, 6-isomer
Terminal alkyne for copper-catalyzed Click chemistry. Tetramethylrhodamine (TAMRA) is a rhodamine dye which is popular in qPCR and other applications. TAMRA is a also a FRET acceptor for FAM.General properties
| Appearance: | red powder |
| Molecular weight: | 530.14 |
| Molecular formula: | C33H40ClN3O |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), , practically insoluble in water (50 mg/L = 80 uM) |
| Quality control: | NMR 1H (95%), HPLC-MS |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
- Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, 2017, 292(11), 4499–4518. doi: 10.1074/jbc.M116.726752
- Ponomarenko, A.I.; Brylev, V.A.; Sapozhnikova, K.A.; Ustinov, A.V.; Prokhorenko, I.A.; Zatsepin, T.S.; Korshun, V.A. Tetrahedral DNA conjugates from pentaerythritol-based polyazides. Tetrahedron, 2016, 72(19), 2386–2391. doi: 10.1016/j.tet.2016.03.051
- Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
Cy2® is a trademark of GE Healthcare.
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