Cyanine3 alkyne
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
A10B0 | 1 mg | $125 | in stock | |
B10B0 | 5 mg | $260 | in stock | |
C10B0 | 10 mg | $325 | in stock | |
D10B0 | 25 mg | $510 | in stock | |
E10B0 | 50 mg | $895 | in stock | |
F10B0 | 100 mg | $1490 | in stock |
Cyanine3 alkyne is a bright and photostable fluorophore for сlick сhemistry, an analog of Cy3®. This dye can be attached to nearly any molecule bearing azide group via copper-catalyzed click-reaction.
This alkyne is non-water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins or peptides.
Cyanine3 absorbance and emission spectra
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DAPI, blue fluorescent nucleic acid stain
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent DNA dye used for chromosome staining and as a nuclear counterstain in fluorescence microscopy and flow cytometry.General properties
Appearance: | red powder |
Molecular weight: | 530.14 |
Molecular formula: | C33H40ClN3O |
Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), , practically insoluble in water (50 mg/L = 80 uM) |
Quality control: | NMR 1H (95%), HPLC-MS |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 555 |
ε, L⋅mol−1⋅cm−1: | 150000 |
Emission maximum, nm: | 570 |
Fluorescence quantum yield: | 0.31 |
CF260: | 0.04 |
CF280: | 0.09 |
Product citations
- Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Scientific Reports, 2021, 11(1), 12229. doi: 10.1038/s41598-021-91612-6.
- Guarin, M.; Ny, A.; De Croze, N.; Maes, J.; Léonard, M.; Annaert, P.; de Witte, P. A. M. Pharmacokinetics in Zebrafish Embryos (ZFE) Following Immersion and Intrayolk Administration: A Fluorescence-Based Analysis. Pharmaceuticals, 2021, 14(6), 576. doi: 10.3390/ph14060576
- Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal imaging and pharmacokinetics of fluorescent compounds in zebrafish eleuthero-embryos after different routes of administration. Scientific Reports, 2021, 11, 12229. doi: 10.1038/s41598-021-91612-6
- Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
Cy2® is a trademark of GE Healthcare.
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