TODi-3, far-red fluorescent nucleic acid stain

Cat. # Quantity Price Lead time
A2610 50 uL, 5 mM/DMSO $85 in stock
22610 200 uL, 5 mM/DMSO $320 in stock
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TODi-3 (Thiazole Red Homodimer, also known as TOTO®-3) is a far-red fluorescent carbocyanine dimeric dye. TODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make TODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. TODi-3 is non-cytotoxic and may be used for long-term monitoring of cell viability and dead cell detection in culture. The long-wavelength fluorescence of TO-TAP-3 is well separated from green and red fluorophores, which makes it ideal for multicolor fluorescence labeling experiments.

Absorption and emission spectra of TODi-3 (DNA–dye complex)

Absorption and emission spectra of TODi-3 (DNA–dye complex)

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General properties

Appearance: blue solution
Molecular weight: 1354.87
CAS number: 166196-17-4
Molecular formula: C53H62I4N6S2
IUPAC name: Quinolinium, 1,1′-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[3-(3-methyl-2(3H)-benzothiazolylidene)-1-propenyl]-, tetraiodide
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 644
Emission maximum, nm: 662
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