DusQ® 3 CPG 500
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1618-100mg | 100 mg | – | in stock | |
| 1618-1g | 1 g |
$990
|
in stock |
DusQ® 3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.
The dye can be attached to 3′-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.
Absorption spectrum of DusQ 3
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Universal CPG type II, 500A
This universal support is intended for automated synthesis of oligonucleotides for short-term dephosphorylation process during deblocking. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.FAM phosphoramidite, 6-isomer
FAM (fluorescein) phosphoramidite 6-isomer, is a widely used reagent in nucleic acid chemistry for labeling oligonucleotides. It is derived from the single pure isomer of 6-carboxyfluorescein and separated from amidite linkage via C6 aminohexanol spacer.General properties
| Appearance: | dark blue beads |
| Quality control: | 1H NMR of bound reagent, coupling testing with HPLC-MS |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 657 |
| ε, L⋅mol−1⋅cm−1: | 45500 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50-80 |
| Coupling conditions: | standard, ultramild phosphoramidites |
| Cleavage conditions: | 2 h / rt, 30% aq. ammonia (ultramild protective groups) |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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