Biotin-11-dUTP

PCR and qPCR Triphosphates
Cat. # Quantity Price Lead time
1553-250nmol 250 nmol –   21 days
1553-500nmol 500 nmol $190.00 21 days
1553-1umol 1 umol $290.00 21 days
1553-5umol 5 umol please inquire 21 days
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Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation, polymerase chain reaction (PCR), random priming, and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.

«11» in the compound name indicates the length of the linker between dUTP and biotin; that linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.

Structure of Biotin-11-dUTP
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Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

FAM-11-dUTP, 6-isomer

FAM-derivative of dUTP for DNA labeling with emission in the green spectrum.
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Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.

General properties

Appearance: colorless solid
Molecular weight: 881.48
Molecular formula: C28H43N6Li3O17P3S
IUPAC name: ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.
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