SIMA-dT phosphoramidite, 6-isomer

Cat. # Quantity Price Lead time
2950-100mg 100 mg $180 in stock
2950-250mg 250 mg $390 in stock
2950-1g 1 g $1120 in stock
2950-5g 5 g $4520 21 days
2950-10g 10 g $8650 in stock
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SIMA-dT phosphoramidite is used to introduce SIMA in the sequence during oligonucleotide synthesis, usually as a substitute for the native dT linkage.

SIMA is known to be much more stable than HEX in basic media thus deprotection in harsh conditions using ammonium hydroxide (up to 6-8 hours at 55 °C) is possible as well as AMA at room temperature or at 65 °C.

Recommendations for using the reagent:

Coupling: 6 minutes coupling time recommended.

Deprotection: standard method recommended, can be deprotected with AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine).

Absorption and emission spectra of SIMA

Absorption and emission spectra of SIMA

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General properties

Appearance: white powder
Molecular weight: 1646.67
Molecular formula: C91H95Cl2N6O17P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 531
ε, L⋅mol−1⋅cm−1: 92300
Emission maximum, nm: 555
Fluorescence quantum yield: 0.63
CF260: 0.57
CF280: 0.18

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: identical to protected nucleobases
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