SynaptoProbe® Red

Cat. # Quantity Price Lead time
3425-100ug 100 ug
– 
in stock
3425-1mg 1 mg
$132
in stock
3425-5mg 5 mg
$350
in stock
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SynaptoProbe® Red is an analog of FM®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.

This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.

SynaptoProbe® Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

Absorption and emission spectra of SynaptoProbe Red

Absorption and emission spectra of  SynaptoProbe Red

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General properties

Appearance: dark powder
Molecular weight: 701.51
CAS number: 162112-35-8 (bromide salt)
Molecular formula: C30H45I2N3
IUPAC name: 4-{6-[4-(diethylamino)phenyl]hexa-1,3,5-trien-1-yl}-1-[3-(triethylammonio)propyl]pyridinium diiodide
Solubility: good in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 536
Emission maximum, nm: 736
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