SynaptoProbe Red

Cat. # Quantity Price Lead time
3425-100ug 100 ug –   in stock
3425-1mg 1 mg $132 in stock
3425-5mg 5 mg $350 in stock
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SynaptoProbe Red is an analog of FM®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.

This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.

SynaptoProbe Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

Absorption and emission spectra of SynaptoProbe Red

Absorption and emission spectra of  SynaptoProbe Red

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General properties

Appearance: dark powder
Molecular weight: 700.51
CAS number: 162112-35-8 (bromide salt)
IUPAC name: 4-{6-[4-(diethylamino)phenyl]hexa-1,3,5-trien-1-yl}-1-[3-(triethylammonio)propyl]pyridinium diiodide
Solubility: good in water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 536
Emission maximum, nm: 736
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