Cat. # Quantity Price Lead time
3079-1mg 1 mg $120.00 in stock
3079-5mg 5 mg $290.00 in stock

This fluorescent conjugate is useful to construct streptavidin-based fluorescent assays and visualize binding of biomolecules. This is especially useful because streptavidin has four binding centers for biotin - this gives opportunity to build different kinds of assays. For example, target molecule having affinity to surface can be conjugated with biotin and be immobilized on solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with biotin-dye conjugate.

The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.

Absorption and emission spectra of sulfo-Cyanine3

Absorption and emission spectra of sulfo-Cyanine3

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Conjugate of biotin with sulfo-Cyanine5 emitting in the far-red region of the spectrum.

General properties

Appearance: dark colored solid
Molecular weight: 1011.32
Molecular formula: C46H63N6KO11S3
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
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