sulfo-Cyanine3-PEG3-biotin

Cat. # Quantity Price Lead time
3079-1mg 1 mg $120 in stock
3079-5mg 5 mg $290 in stock
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This fluorescent conjugate is useful for streptavidin-based fluorescent assays and visualization of the streptavidin/avidin-labeled biomolecules. Streptavidin, a protein with a high affinity to biotin, has four binding centers for biotin. The high stability of biotin/streptavidin complex gives the opportunity to build different kinds of assays. For example, a target molecule with affinity to the surface can be conjugated with biotin and immobilized on a solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with the biotin-dye conjugate.

The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.

Absorption and emission spectra of sulfo-Cyanine3

Absorption and emission spectra of sulfo-Cyanine3

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General properties

Appearance: dark colored solid
Molecular weight: 1011.32
Molecular formula: C46H63N6KO11S3
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
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