FAM tetrazine, 5-isomer

Cat. # Quantity Price Lead time
141E0 1 mg $110.00 in stock
241E0 5 mg $210.00 in stock
441E0 25 mg $410.00 in stock
541E0 50 mg $695.00 in stock
641E0 100 mg $1190.00 in stock

Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.

Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: yellow solid
Mass spec M+ increment: 529.12
Molecular weight: 559.53
Molecular formula: C31H21N5O6
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17
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