Sulfo-Cyanine5.5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17380 | 1 mg | $110.00 | in stock | |
| 27380 | 5 mg | $290.00 | in stock | |
| 47380 | 25 mg |
$690.00
|
in stock | |
| 57380 | 50 mg |
$1270.00
|
in stock | |
| 67380 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine5.5 dye is a water soluble far red to NIR emitting dye which is very hydrophilic due to the presence of four sulfo groups. Sulfo-Cyanine5.5 derivatives exhibit high water solubility. The dye is a perfect choice for the labeling of sensitive proteins, nanoparticles, and highly hydrophylic biopolymers.
This maleimide is a thiol reactive dye that selectively labels cysteine residues. Disulfide bonds of native proteins should be reduced with an appropriate reducing agent, such as TCEP, according to our recommended protocol.
Absorption and emission spectra of sulfo-Cyanine5.5
Recommended protocol
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Sulfo-Cyanine3 maleimide
Water soluble thiol-reactive derivative of Sulfo-Cyanine3, a Cy3 analog.sulfo-Cyanine5.5 DBCO
Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free reaction with azides.BDP FL maleimide
BDP FL maleimide is a bright and photostable thiol-reactive dye for protein labeling, an ideal replacement for fluorescein for microscopy.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 1024.2 |
| Molecular weight: | 1139.43 |
| CAS number: | 2183440-58-4 (potassium salt); 2183440-57-3 (inner salt) |
| Molecular formula: | C46H45K3N4O15S4 |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 673 |
| ε, L⋅mol−1⋅cm−1: | 211000 |
| Emission maximum, nm: | 691 |
| Fluorescence quantum yield: | 0.21 |
| CF260: | 0.09 |
| CF280: | 0.11 |
Product citations
- Ieong, K.-W.; Indrisiunaite, G.; Prabhakar, A.; Puglisi, J.D.; Ehrenberg, M. N6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors. Nucleic Acids Research, 2021, 49(5), 2684–2699. doi: 10.1093/nar/gkab033
- Chen, Q.; Gao, M.; Li, Z.; Xiao, Y.; Bai, X.; Boakye-Yiadom, K.O.; Xu, X.; Zhang, X.-Q. Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy. Journal of Controlled Release, 2020, 323, 179–190. doi: 10.1016/j.jconrel.2020.03.044
- Wang, J.; Johnson, A.G.; Lapointe, C.P.; Choi, J.; Prabhakar, A.; Chen, D.-H.; Petrov, A.N.; Puglisi, J.D. eIF5B gates the transition from translation initiation to elongation. Nature, 2019, 573, 605–608. doi: 10.1038/s41586-019-1561-0
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