Sulfo-Cyanine5.5 maleimide
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17380
1 mg
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5 mg
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25 mg
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50 mg
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67380
100 mg
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Sulfo-Cyanine5.5 dye is a water soluble far red to NIR emitting dye which is very hydrophilic due to the presence of four sulfo groups. Sulfo-Cyanine5.5 derivatives exhibit high water solubility. The dye is a perfect choice for the labeling of sensitive proteins, nanoparticles, and highly hydrophylic biopolymers.
This maleimide is a thiol reactive dye that selectively labels cysteine residues. Disulfide bonds of native proteins should be reduced with an appropriate reducing agent, such as TCEP, according to our recommended protocol.
Absorption and emission spectra of sulfo-Cyanine5.5
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Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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General properties
Appearance:
dark colored solid
Mass spec M+ increment:
1024.2
Molecular weight:
1139.43
CAS number:
2183440-58-4 (potassium salt); 2183440-57-3 (inner salt)
Molecular formula:
C46 H45 K3 N4 O15 S4
Solubility:
good in water, DMSO, DMF
Quality control:
NMR 1 H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
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Product specifications
Spectral properties
Excitation maximum, nm:
673
ε, L⋅mol−1 ⋅cm−1 :
235000
Emission maximum, nm:
691
CF260 :
0.09
CF280 :
0.11
Product citations
Chen, Q.; Gao, M.; Li, Z.; Xiao, Y.; Bai, X.; Boakye-Yiadom, K.O.; Xu, X.; Zhang, X.-Q. Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy. Journal of Controlled Release , 2020 , 323, 179–190. doi: 10.1016/j.jconrel.2020.03.044
Wang, J.; Johnson, A.G.; Lapointe, C.P.; Choi, J.; Prabhakar, A.; Chen, D.-H.; Petrov, A.N.; Puglisi, J.D. eIF5B gates the transition from translation initiation to elongation. Nature , 2019 , 573, 605–608. doi: 10.1038/s41586-019-1561-0
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