sulfo-Cyanine5.5 maleimide

Cat. # Quantity Price Lead time
17380 1 mg $125 in stock
27380 5 mg $325 in stock
47380 25 mg $790 in stock
57380 50 mg $1490 in stock
67380 100 mg $1990 in stock
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sulfo-Cyanine5.5 dye is a water-soluble far-red to NIR emitting dye that is very hydrophilic due to the presence of four sulfo groups. sulfo-Cyanine5.5 derivatives exhibit high water solubility. The dye is perfect for labeling sensitive proteins, nanoparticles, and highly hydrophilic biopolymers.

This maleimide is a thiol-reactive dye that selectively labels cysteine residues. Disulfide bonds of native proteins should be reduced with an appropriate reducing agent, such as TCEP, according to our recommended protocol.

Absorption and emission spectra of sulfo-Cyanine5.5

Absorption and emission spectra of sulfo-Cyanine5.5

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sulfo-Cyanine5.5 azide

sulfo-Cyanine5.5 azide is a derivative of water-soluble far-red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper-free click chemistry with alkynes and cycloalkynes.

General properties

Appearance: dark colored solid
Mass spec M+ increment: 1024.2
Molecular weight: 1139.43
CAS number: 2183440-58-4 (potassium salt); 2183440-57-3 (inner salt)
Molecular formula: C46H45K3N4O15S4
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 673
ε, L⋅mol−1⋅cm−1: 211000
Emission maximum, nm: 691
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.11

Product citations

  1. Sabuncu, S.; Montoya Mira, J.; Quentel, A.; Gomes, M. M.; Civitci, F.; Fischer, J. M.; Yildirim, A. Protein-Coated Biodegradable Gas-Stabilizing Nanoparticles for Cancer Therapy and Diagnosis Using Focused Ultrasound. Advanced Materials Interfaces, 2023, 10(2), 2201543. doi: 10.1002/admi.202201543
  2. Das, S.K.; Huynh, M.T.; Lee, T.-H. Spontaneous histone exchange between nucleosomes. The Journal of Biological Chemistry, 2023, 299(8), 105037. doi: 10.1016/j.jbc.2023.105037
  3. Çetin, B.; O’Leary, S.E. mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation. Nucleic Acids Research, 2022, 50(14), 8240-8261. doi: 10.1093/nar/gkac631
  4. Ieong, K.-W.; Indrisiunaite, G.; Prabhakar, A.; Puglisi, J.D.; Ehrenberg, M. N6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors. Nucleic Acids Research, 2021, 49(5), 2684–2699. doi: 10.1093/nar/gkab033
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