sulfo-Cyanine5.5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17380 | 1 mg | $125 | in stock | |
| 27380 | 5 mg | $325 | in stock | |
| 47380 | 25 mg |
$790
|
in stock | |
| 57380 | 50 mg |
$1490
|
in stock | |
| 67380 | 100 mg |
$1990
|
in stock |
sulfo-Cyanine5.5 dye is a water-soluble far-red to NIR emitting dye that is very hydrophilic due to the presence of four sulfo groups. sulfo-Cyanine5.5 derivatives exhibit high water solubility. The dye is perfect for labeling sensitive proteins, nanoparticles, and highly hydrophilic biopolymers.
This maleimide is a thiol-reactive dye that selectively labels cysteine residues. Disulfide bonds of native proteins should be reduced with an appropriate reducing agent, such as TCEP, according to our recommended protocol.
Absorption and emission spectra of sulfo-Cyanine5.5
Recommended protocol
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sulfo-Cyanine5 maleimide
Thiol reactive maleimide, water soluble cyanine dye for Cy5 channel.Cyanine7 NHS ester
Amine reactive NHS ester of near infrared dye Cyanine7.Pentynoic acid STP ester
An activated ester for the introduction of alkyne into peptides/proteins and click reaction.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 1024.2 |
| Molecular weight: | 1139.43 |
| CAS number: | 2183440-58-4 (potassium salt); 2183440-57-3 (inner salt) |
| Molecular formula: | C46H45K3N4O15S4 |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 673 |
| ε, L⋅mol−1⋅cm−1: | 211000 |
| Emission maximum, nm: | 691 |
| Fluorescence quantum yield: | 0.21 |
| CF260: | 0.09 |
| CF280: | 0.11 |
Product citations
- Sabuncu, S.; Montoya Mira, J.; Quentel, A.; Gomes, M. M.; Civitci, F.; Fischer, J. M.; Yildirim, A. Protein-Coated Biodegradable Gas-Stabilizing Nanoparticles for Cancer Therapy and Diagnosis Using Focused Ultrasound. Advanced Materials Interfaces, 2023, 10(2), 2201543. doi: 10.1002/admi.202201543
- Das, S.K.; Huynh, M.T.; Lee, T.-H. Spontaneous histone exchange between nucleosomes. The Journal of Biological Chemistry, 2023, 299(8), 105037. doi: 10.1016/j.jbc.2023.105037
- Çetin, B.; O’Leary, S.E. mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation. Nucleic Acids Research, 2022, 50(14), 8240-8261. doi: 10.1093/nar/gkac631
- Ieong, K.-W.; Indrisiunaite, G.; Prabhakar, A.; Puglisi, J.D.; Ehrenberg, M. N6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors. Nucleic Acids Research, 2021, 49(5), 2684–2699. doi: 10.1093/nar/gkab033
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