sulfo-Cyanine5.5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17380 | 1 mg | $125.00 | in stock | |
| 27380 | 5 mg | $325.00 | 5 days | |
| 47380 | 25 mg |
$790.00
|
in stock | |
| 57380 | 50 mg |
$1490.00
|
in stock | |
| 67380 | 100 mg |
$1990.00
|
in stock |
sulfo-Cyanine5.5 dye is a water-soluble far-red to NIR emitting dye that is very hydrophilic due to the presence of four sulfo groups. sulfo-Cyanine5.5 derivatives exhibit high water solubility. The dye is perfect for labeling sensitive proteins, nanoparticles, and highly hydrophilic biopolymers.
This maleimide is a thiol-reactive dye that selectively labels cysteine residues. Disulfide bonds of native proteins should be reduced with an appropriate reducing agent, such as TCEP, according to our recommended protocol.
Absorption and emission spectra of sulfo-Cyanine5.5
Recommended protocol
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sulfo-Cyanine3 maleimide
Water soluble thiol-reactive derivative of sulfo-Cyanine3, a Cy3 analog.FAM amine, 6-isomer
Amine derivative of fluorescein (FAM) fluorophore, pure 6-isomer, for the enzymatic transamination labeling, reactions with electrophilic reagents, and reductive amination.General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 1024.2 |
| Molecular weight: | 1139.43 |
| CAS number: | 2183440-58-4 (potassium salt); 2183440-57-3 (inner salt) |
| Molecular formula: | C46H45K3N4O15S4 |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 673 |
| ε, L⋅mol−1⋅cm−1: | 211000 |
| Emission maximum, nm: | 691 |
| Fluorescence quantum yield: | 0.21 |
| CF260: | 0.09 |
| CF280: | 0.11 |
Product citations
- Sabuncu, S.; Montoya Mira, J.; Quentel, A.; Gomes, M. M.; Civitci, F.; Fischer, J. M.; Yildirim, A. Protein-Coated Biodegradable Gas-Stabilizing Nanoparticles for Cancer Therapy and Diagnosis Using Focused Ultrasound. Advanced Materials Interfaces, 2023, 10(2), 2201543. doi: 10.1002/admi.202201543
- Ieong, K.-W.; Indrisiunaite, G.; Prabhakar, A.; Puglisi, J.D.; Ehrenberg, M. N6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors. Nucleic Acids Research, 2021, 49(5), 2684–2699. doi: 10.1093/nar/gkab033
- Chen, Q.; Gao, M.; Li, Z.; Xiao, Y.; Bai, X.; Boakye-Yiadom, K.O.; Xu, X.; Zhang, X.-Q. Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy. Journal of Controlled Release, 2020, 323, 179–190. doi: 10.1016/j.jconrel.2020.03.044
- Duss, O.; Stepanyuk, G.A.; Puglisi, J.D.; Williamson, J.R. Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Cell, 2019, 179, 1357–1369.e16. doi: 10.1016/j.cell.2019.10.035
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