Sulfo-Cyanine3.5 NHS ester

Cat. # Quantity Price Lead time
12320 1 mg $110.00 in stock
22320 5 mg $290.00 in stock
42320 25 mg $690.00 in stock
52320 50 mg $1270.00 in stock
62320 100 mg $1990.00 in stock

Cyanine3.5 is a fluorescent dye with absorption and emission intermediate between Cyanine3 and Cyanine5. Sulfonated version contains four sulfo groups which ensure high solubility and hydrophilicity of the fluorophore and labeled conjugates.

The NHS ester reactive group allows to label primary and secondary amine groups.

Absorption and emission spectra of sulfo-Cyanine3.5

Absorption and emission spectra of sulfo-Cyanine3.5

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AF488 NHS ester

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Sulfo-Cyanine3.5 alkyne

Alkyne derivative of water-soluble Sulfo-Cyanine3.5 dye for copper-catalyzed Click chemistry (CuAAC).

General properties

Appearance: deep purple powder
Molecular weight: 1088.33
CAS number: 2231670-91-8 (without cation)
Molecular formula: C42H40N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17

Product citations

  1. Shramova, E.; Proshkina, G.; Shipunova, V.; Ryabova, A.; Kamyshinsky, R.; Konevega, A.; Schulga, A.; Konovalova, E.; Telegin, G.; Deyev, S. Dual Targeting of Cancer Cells with DARPin-Based Toxins for Overcoming Tumor Escape. Cancers, 2020, 12(10), 3014. doi: 10.3390/cancers12103014
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