AF 546 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1F820 | 1 mg |
$135.00
|
in stock | |
| 2F820 | 5 mg |
$395.00
|
in stock | |
| 4F820 | 25 mg |
$1289.00
|
12 days | |
| 5F820 | 50 mg |
$1860.00
|
12 days | |
| 6F820 | 100 mg |
$2490.00
|
7 days |
AF 546 NHS ester is a rhodamine-based fluorescent dye designed for covalent labeling of biomolecules at primary amines.
AF 546 NHS ester effectively reacts with the ε-amino groups of lysines and the N-terminal amino groups of proteins, forming a stable amide bond. The reaction is carried out in a slightly alkaline environment (typically pH 7.5–8.5), which ensures high conjugation and reproducible results.
The dye is characterized by high brightness, good photostability, and minimal pH sensitivity in the physiological range. Its spectral properties make it suitable for multicolor fluorescence microscopy, flow cytometry, and fluorescence immunoassays.
Absorption and emission spectra of AF 546
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General properties
| Appearance: | dark-red crystals |
| Molecular weight: | 1159.63 |
| Molecular formula: | C50H62Cl3N5O14S3 |
| Solubility: | water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 130000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.25 |
| CF280: | 0.13 |
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